为建立我国含脂食品辐照鉴定方法提供技术支持,以^60Co-γ射线为辐照源,采用不同剂量(1、3、5、7kGy)辐照猪肉,对辐照后的猪肉进行索氏提取脂肪,固相萃取净化所提油脂,去除其中的色素和脂肪。氮气吹干碳氢化合物滤液,并用正己烷定容到1mL。用GC-MS检测收集液中的挥发性碳氢化合物的种类和含量。结果表明:辐照后猪肉中的碳氢化合物的种类比辐照之前多了两种,它们分别为C16:2和C17:1,而且它们的峰强度随着辐照剂量的增加而提高;另外,空白样品中各种脂肪酸含量的高低对碳氢化合物的含量也有影响。
In order to establish a method for identifying radiated pork based on differences in the contents of hydrocarbons, pork was radiated by ^60Coγ ray at different dosages (1, 3, 5 kGy and 7 kGy) and radiated pork was analyzed for kinds and contents of volatile hydrocarbons in it by GC-MS based on a sample preparation procedure consisting of Soxhlet extraction, cleanup by solid-phase extraction for the removal of pigments and fat, nitrogen blowing evaporation to dryness and dilution in n-hexane to 1 mL. It was found that ~(60)Coγ ray radiation resulted in the generation of two new compounds in pork, namely C16:2 and C17:1. and that their contents both increased with increasing radiation dosage. Moreover, after ~(60)Coγ ray radiation at higher dosages, more hydrocarbons were found in pork with higher fatty acid contents.