东篱科研大数据发现系统（DRDS）

位置：成果数据库 > 期刊 > 期刊详情页

秋水仙碱对微管蛋白的作用机制及其细胞效应研究进展

期刊名称：西北植物学报
时间：0
页码：2570-2578
语言：中文
分类：S512.101[农业科学—作物学] X16[环境科学与工程—环境科学]
作者机构：[1]National Engineering Laboratory for Forest Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, P. R. China, [2]Department of Biology and Chemistry, Baoding University, Baoding 071000, E R. China, [3]Beijing Institute of Landscape Architecture, Beijing 100102, P. R. China, [4]Hardwood Tree Improvement and Regeneration Center （HTIRC）, Department of Forestry and Natural Resources, Purdue University, West Lafayette, Indiana 47907, USA
相关基金：Acknowledgements We thank Mr. Shen Xin of the College of Biological Sciences and Biotechnology, Beijing Forestry University, who gave many valuable comments and suggestions in test programs and research work. We thank the support from the National Science Foundation of China （Grant Nos. 30771747 and 30640036）, the Beijing Natural Science Foundation （No. 6042020）, the research projects supported by the Hebei Education Department （No. Z2010102） and the Baoding University （No. 2010Z02）.
相关项目：银杏2n花粉秋水仙素诱导率极低的微管及微管蛋白机理研究

关键词：线粒体DNA多态性, 叶绿体DNA, PCR扩增, 遗传, 技术检测, 杂交组合, mtDNA, DNA片段, Section Leuce, chloroplast DNA, mitochondrial DNA, polymorphism, heredity

中文摘要：

叶绿体 DNA (cpDNA ) 和 16 Populus 种类(节 Leuce ) 和他们的 F1 产生的 mitochondrial DNA (mtDNA ) 用 PCR-RFLP 技术被检测。结果证明在 22 混合联合的 F1 产生的 cpDNA 是继承似母亲，它支持了 plasmid 细胞学的学习的结论。PCR 放大的 mtDNA 碎片与在所有混合联合的限制地图一致，没有多型性被检测，显示节 Leuce 高度在 mitochondrial 基因序列被保存。提供的这些结果在 Populus tomentosa 指导母亲的叶绿体继承的证据， P。bolleana， P。davidiana， P。adenopoda， P。tomentosa

英文摘要：

The chloroplast DNA （cpDNA） and mitochondrial DNA （mtDNA） of 16 Populus species （Section Leuce） and their F1 generation were detected using PCR-RFLP technique. The results show that cpDNA in the F1 generation of 22 hybrid combinations was inherited maternally, which supported the conclusions of the study of plasmid cytology. The mtDNA fragments amplified by PCR were consistent with the restriction maps in all hybrid combinations and no polymorphism was detected, indicating that the Section Leuce is highly conserved in mitochondrial gene sequences. These results provided direct evidence of maternal chloroplast inheritance in Populus tomentosa, P. bolleana, P. davidiana, P. adenopoda, P. tomentosa × P. bolleana, P. alba × P. glandulosa and P. alba × P. tomentosa.

同期刊论文项目