为了应用电子自旋共振(ESR)波谱技术检测辐照葡萄,以葡萄皮、葡萄柄和葡萄籽为试验材料,研究其在0~10.0kGy剂量范围ESR波谱特征变化以及辐照剂量与信号强度的关系。结果表明:葡萄皮、葡萄柄和葡萄籽辐照后ESR波谱均有明显的区别,信号强度均随辐照剂量增加而增大。葡萄柄的辐照剂量检出限最低(0.25kGy),是鉴定葡萄样品是否经过辐照的理想材料。通过比较葡萄皮、葡萄柄和葡萄籽剂量效应曲线得出葡萄柄拟合曲线最为准确(R2=0.9943)。3种辐照试验材料在贮藏期(15d)内信号强度均有不同程度的衰减,辐照葡萄皮信号强度衰减最为剧烈(衰减80%)。研究结果为ESR波谱技术在辐照葡萄检测中的应用提供了技术依据。
In order to detect irradiated grapes by electron spin resonance(ESR)spectroscopy,the grape skins,grape stalks and grape seeds were used as test materials to study the feature changes of ESR spectrum and the relationship between ESR intensity and irradiation dose in the range of 0 to 10 kGy.The results showed that the ESR spectra of grape skins,grape stalks and grape seeds were obviously different before and after irradiation,the intensity of ESR signal increased with the increasing of the absorbed dose.The grape stalks which had the minimum detection limit(0.25 kGy)could be used as an ideal experimental material to identify whether or not grapes had been irradiated.By comparing the dose effect curves of grape skins,grape stalks and grape seeds,it was concluded that grape stalk curves showed the most accurate(R 2 =0.9943).The ESR intensity of three kinds of irradiated samples all decreased during the storage time(15 d),grape skins showed severest attenuation(attenuation 80%).The results could provide the technical basis for the application of ESR spectroscopy in detecting irradiated grapes.