DNA双链断裂(DSBs)是细胞最严重的损伤形式之一。高等动植物中主要通过非同源末端连接(NHEJ)途径进行DNA双链断裂修复。该途径不依赖DNA同源性,由一些修复因子如:Ku蛋白异二聚体、DNA-PKcs、XRCC4和ligaseⅣ等,将断裂末端直接连接进行修复。综述了植物DNA双链断裂损伤修复的主要途径及其相关基因研究的进展,探讨了植物DNA损伤修复研究中存在的问题与发展方向。
The DNA double-strand breaks(DSBs) is one of the most serious form of DNA damage.Non-homologous end-joining(NHEJ) is the major pathway to repair DNA double-strand break damages in both higher plants and animals.The NHEJ pathway does not rely on DNA homology,while the number of such repair factors as Ku protein heterodimer,DNA-PKcs,XRCC4 and ligaseⅣ,will be directly joined the end to repair.The research progresses in major repair pathway and key genes involving in DNA double-strand break damages in plant are summarized.The existing problems and perspectives in this field are also discussed.