为研究外源豌豆铁蛋白基因(Pea-Fer)的导入和表达对水稻植株和子粒重要矿质元素的积累所产生的影响,本试验以原始受体亲本粳稻品种秀水11为轮回亲本,外源豌豆铁蛋白转基因纯系Fer34为非轮回亲本,采取连续回交,结合GUS标记基因辅助选择技术,在BC6F3获得含Pea-Fer的Fer34-XS11,它与秀水11构成一对近等基因系。并利用该近等基因系,研究Pea-Fer基因导入对水稻植株不同生长发育阶段(幼苗期、分蘖期、成熟期)的根、茎(叶鞘)、叶及子粒不同部分(谷壳、米糠、糙米、精米)的主要矿质元素(Fe、Ca、Mn、Zn)积累的影响。结果表明:外源豌豆铁蛋白基因(Pea-Fer)的导入,使得水稻植株在不同生长发育时期的根、茎(叶鞘)、叶等器官中的Fe含量较对照品种秀水11明显增加,而对于Ca、Mn和Zn的含量并没有显著影响;同时,水稻子粒中Fe含量积累也有较高提升,而Ca、Mn和Zn的积累却未出现显著变化。这为深入开展转基因富铁水稻新种质的研究和利用提供了一定依据。
To investigate the effect of exogenous pea ferritin gene(Pea-Fer)on major mineral elements accumulation in transgenic rice,a near isogenic line(NIL BC6F3)Fer34-XS11 was developed from the donor parent(exogenous ferritin transgenic pure line Fer34)and the recurrent parent(Oryza sativa ssp.japanica)(Xiushui 11)through repeated backcrossing and self-crossing.The contents of major mineral elements,including Fe,Ca,Mn and Zn,from various tissues(root,stem and leaf sheath)at different developmental stages(seedling,tillering and maturing)of the transgenic plants and grains were further analyzed.The results showed that the iron content of Fer34-XS11 increased significantly in different organs,developmental stages and grains,but no obvious difference was observed in other mineral elements(Ca,Mn and Zn)compared to the wild type Xiushui 11.This result provides important information for further studying and utilizing the new iron-rich rice.