中文摘要：

目的：评价样本保存时间及温度对流式细胞仪检测非健康人外周血淋巴细胞亚群的影响。方法第1组，随机选取患者27例，分别在采血后6 h内（A组）、在4℃保存72 h （B组）和120 h （C组）后进行检测。第2组，随机选取患者18例，分别在采血后6 h内检测（D组）；在采血后6 h内预处理，4℃保存24 h后检测（E组）；在室温25℃保存36 h后预处理，随后检测（F组）。结果第1组，与A组相比，B组和C组的CD3＋细胞、CD3＋ CD4＋细胞明显降低，而CD19＋细胞及CD16＋CD56＋细胞明显升高，差异均有统计学意义（ P＜0．05）；B组的CD3＋ CD8＋细胞明显升高（P＜0．05）。第2组，D组、E组和F组之间结果比较，差异无统计学意义（P＞0．05）。结论在标本采集后36 h内对非健康人的样本进行淋巴细胞亚群检测，结果稳定。

英文摘要：

Objective To evaluate the effect of sample storage time and temperature on the analysis of periph‐eral blood lymphocyte subsets in non‐healthy human by flow cytometry .Methods Group one involved 27 patients with multiple diseases .Whole blood was collected for flow cytometry analysis within 6 h（group A） ,at 72 h（group B） and 120 h（group C） .Group two involved 18 patients with multiple diseases .Samples were tested in 3 groups：group D ,the samples were processed and tested within 6 hours since it collected;group E ,samples were processed within 6 hours ,and were stored at 4 ℃ for 24 hours before test ;group F ,samples were stored at 25 ℃ for 36 hours ,and then were processed and tested .Results Compared with group A in group one ,the data showed lower CD3＋ cells ,CD3＋CD4＋ cells（P〈0 .05）and higher CD19＋ cells（P〈0 .05） ,CD16＋ CD56＋ cells（P〈 0 .05）of group B and group C . CD3＋CD8＋ cells were higher in group B than group A（P〈0 .05） .In group two ,there was no significant difference between group D ,group E and group F（P〉0 .05） .Conclusion The results of peripheral blood lymphocyte subsets of patients analyzed by flow cytometry are stable within 36 hours after the sample collection .