中文摘要：

目的探讨宫颈病变中增殖细胞核抗原（PCNA）、Ki67抗原（Ki-67）的表达及其与人乳头瘤病毒（HPV）感染的相关性分析。方法选取2012年4月～2014年4月该院手术切除的85例宫颈标本为研究对象，其中宫颈上皮内瘤变（CIN）43例、慢性宫颈炎19例、子宫颈浸润性鳞癌23例；再选取正常宫颈组织20例，比较不同宫颈病变组织中的PCNA以及Ki-67免疫组化结果并进行相关分析，并结合患者HPV检验结果进行比较。结果PCNA在慢性宫颈炎、CIN及子宫颈浸润性鳞癌中的阳性表达率分别为52．63％、88．37％和100．00％，在CIN以及子宫颈浸润性鳞癌中的PCNA表达率均高于慢性宫颈炎，差异均有统计学意义（P〈0．05）；Ki-67在慢性宫颈炎、CIN及子宫颈浸润性鳞癌中的阳性表达率分别为47．37％、81．40％和100．00％，在CIN以及子宫颈浸润性鳞癌中的Ki-67表达率均高于慢性宫颈炎，差异均有统计学意义（P〈0．05）；PCNA与Ki-67的表达强度呈正相关（r=0．583，P〈0．05）；PCNA的表达强度与HPV感染呈正相关（r=0．645，P〈0．05）；Ki-67的表达强度与HPV感染呈正相关（r=0．603，P〈0．05）。结论PCNA、Ki-67在不同宫颈病变中的阳性表达有差异，且PCNA、Ki-67的表达强度与HPV感染相关，可将PCNA、Ki-67与宫颈HPV感染检测相结合，以提高发现高度病变倾向存在的敏感性，从而提高宫颈病变患者早期诊断的准确率。

英文摘要：

Objective To explore the expressions of proliferating cell nuclear antigen （PCNA） and Ki-67 in cervical lesions and their correlations with human papillomavirus （HPV） infection. Methods Eighty-five resected cervical lesions specimens were selected from the hospital from April 2012 to April 2014, including 43 specimens of cervical intraepithelial neoplasia （CIN）, 19 specimens of chronic cervicitis, and 23 specimens of invasive cervical squamous cell carcinoma; 20 normal cervical specimens were selected; the immunohistochemical results of PCNA and Ki-67 in different cervical specimens were compared and a correlation analysis was conducted, HPV test results of these patients were combined for comparison. Results The positive expression rates of PCNA in chronic cervicitis, CIN, and invasive cervical squamous cell carcinoma were 52. 63%, 88. 37%, 100. 00%, respectively; the positive expression rates of PCNA in CIN and invasive cervical squamous cell carcinoma were statistically significantly higher than that in chronic cervicitis （ P〈0. 05 ） . The positive expression rates of Ki-67 in chronic cervicitis, CIN, and invasive cervical squamous cell carcinoma were 47.37%, 81.40%, and 100. 00% , respectively; the positive expression rates of Ki-67 in CIN and invasive cervical squamous cell carcinoma were statistically signif- icantly higher than that in chronic cervicitis （P〈0. 05） . There was a positive correlation between PCNA expression and Ki-67 expression （r=0. 583, P〈0. 05 ）; there was a positive correlation between PCNA expression and HPV infection （r=0. 645, P〈0. 05 ）; there was a positive correlation between Ki-67 expression and HPV infection （ r = 0. 603, P 〈0. 05 ） . Conclusion The positive expression rates of PCNA and Ki-67 in different cervical lesions are various, the positive expression rates of PCNA and Ki-67 are correlated with HPV infection, PCNA and Ki-67 detection combined with cervical HPV infection detection can improve the sensitivity of finding highly lesions