中文摘要：

目的：观察同种异体皮肤移植家兔注射重组人IL-10后血清IL-17家族水平的变化,探究重组人IL-10抑制免疫反应的机制。方法：30只健康新西兰白兔依据随机平均原则分为A组[重组人IL-10低剂量组,5μg/（kg·d）]、B组[重组人IL-10高剂量组,10μg/（kg·d）]、C组[环孢菌素低剂量组,5mg/（kg·d）]、D组[环孢菌素低剂量组,10mg/（kg·d）]、E组[重组人IL-10低剂量,5μg/（kg·d）＋环孢菌素低剂量组,5mg/（kg·d）]、F组[生理盐水空白对照组,0.9%氯化钠溶液1ml/d]六组,每组5只,均经肌肉注射于皮肤移植术前1d给药,共给药10d,各组分别于术前1d,术后1d、4d、1周、2周、3周、4周取兔耳中动脉血,分离制得血清后,依酶联免疫法测定IL-17家族各成员（IL-17A~F）的浓度。结果：家兔同种异体皮肤移植术后IL-17A、IL-27（IL-17B/C/D）、IL-17E（IL-25）、IL-17F的浓度均高于术前,且在术后2周达到高峰,随后逐渐降低;rhIL-10能抑制IL-17A、IL-27、IL-17F的分泌,且抑制效应随着rhIL-10剂量的增多而加强;rhIL-10可促进IL-25的分泌,但无明显量效关系。结论：同种异体皮肤移植家兔注射rhIL-10后血清IL-17家族水平明显提高,rhIL-10对免疫反应的抑制作用可能与IL-17家族介导的炎性反应及炎性因子分泌有关。

英文摘要：

Objective This study is in order to observe serum IL-17 family level variation of rabbits treated with allogeneic skin grafting after injection of recombinational human IL-10, and also in order to investigate immunoreaction inhibition mechanism of recombinational human IL-10. Methods 30 healthy new zealand white rabbits were randomly and averagely distributed into 6 groups, which included group A（recombinational human IL-10 low-dose group, 5μg /（kg.d））, group B（recombinational human IL-10 high-dose group, 10μg /（kg.d））, group C（cyclosporine low-dose group, 5mg /（kg.d））, group D（cyclosporine highdose group, 10 mg /（kg.d））, group E（recombinational human IL-10 low-dose group, 5μg /（kg.d） cyclosporine low-dose group, 5mg /（kg.d）） and group F（normal saline blank control group, 0.9% sodium chloride solution 1ml/d）, each group had 5 rabbits. These rabbits were continuously treated with drugs by intramuscular injection for 10 days from 1 d before skin grafting operation,middle ear arterial blood was respectively collected for rabbits of all groups on 1d before operation, 1d, 4d, 1 week, 2 weeks,3weeks and 4 weeks after operation. Serum was separated. Enzyme linked immunosorbent assay was used for detection concentration of IL-17 family members（IL-17A-F）. Results Concentration of IL-17 A,IL-27（IL-17B/C/D）,IL-17E（IL-25）,IL-17 F for rabbits after allogeneic skin grafting operation was higher than that before operation, concentration of IL-17 A,IL-27（IL-17B/C/D）,IL-17E（IL-25）,IL-17 F reached to the peak value on 2 weeks after operation, then concentration decreased. rhIL-10 could inhibit secretion of IL-17 A,IL-27 and IL-17 F. inhibiting effect was strengthened with the increase of rhIL-10 dose, rhIL-10 could promote secretion of IL-25,no obvious dose-effect relationship was found. Conclusion Serum IL-17 family level of rabbit treated with allogeneic skin grafting after injection of recombinational human IL-10 was obviously improved,inhibiting effect of r