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同种异体皮肤移植家兔注射重组人IL-10后血清IL-17家族水平的变化观察
  • ISSN号:1008-6455
  • 期刊名称:《中国美容医学》
  • 时间:0
  • 分类:Q813.1[生物学—生物工程]
  • 作者机构:四川医科大学附属中医医院,四川泸州646000
  • 相关基金:人体营养素检测关键技术与产品开发主题项目(编号:2014AA022304); 国家高技术研究发展技术“863”计划:课题名称:高血压患者同型半胱氨酸与肌红蛋白和游离脂肪酸相关性研究
中文摘要:

目的:观察同种异体皮肤移植家兔注射重组人IL-10后血清IL-17家族水平的变化,探究重组人IL-10抑制免疫反应的机制。方法:30只健康新西兰白兔依据随机平均原则分为A组[重组人IL-10低剂量组,5μg/(kg·d)]、B组[重组人IL-10高剂量组,10μg/(kg·d)]、C组[环孢菌素低剂量组,5mg/(kg·d)]、D组[环孢菌素低剂量组,10mg/(kg·d)]、E组[重组人IL-10低剂量,5μg/(kg·d)+环孢菌素低剂量组,5mg/(kg·d)]、F组[生理盐水空白对照组,0.9%氯化钠溶液1ml/d]六组,每组5只,均经肌肉注射于皮肤移植术前1d给药,共给药10d,各组分别于术前1d,术后1d、4d、1周、2周、3周、4周取兔耳中动脉血,分离制得血清后,依酶联免疫法测定IL-17家族各成员(IL-17A~F)的浓度。结果:家兔同种异体皮肤移植术后IL-17A、IL-27(IL-17B/C/D)、IL-17E(IL-25)、IL-17F的浓度均高于术前,且在术后2周达到高峰,随后逐渐降低;rhIL-10能抑制IL-17A、IL-27、IL-17F的分泌,且抑制效应随着rhIL-10剂量的增多而加强;rhIL-10可促进IL-25的分泌,但无明显量效关系。结论:同种异体皮肤移植家兔注射rhIL-10后血清IL-17家族水平明显提高,rhIL-10对免疫反应的抑制作用可能与IL-17家族介导的炎性反应及炎性因子分泌有关。

英文摘要:

Objective This study is in order to observe serum IL-17 family level variation of rabbits treated with allogeneic skin grafting after injection of recombinational human IL-10, and also in order to investigate immunoreaction inhibition mechanism of recombinational human IL-10. Methods 30 healthy new zealand white rabbits were randomly and averagely distributed into 6 groups, which included group A(recombinational human IL-10 low-dose group, 5μg /(kg.d)), group B(recombinational human IL-10 high-dose group, 10μg /(kg.d)), group C(cyclosporine low-dose group, 5mg /(kg.d)), group D(cyclosporine highdose group, 10 mg /(kg.d)), group E(recombinational human IL-10 low-dose group, 5μg /(kg.d) cyclosporine low-dose group, 5mg /(kg.d)) and group F(normal saline blank control group, 0.9% sodium chloride solution 1ml/d), each group had 5 rabbits. These rabbits were continuously treated with drugs by intramuscular injection for 10 days from 1 d before skin grafting operation,middle ear arterial blood was respectively collected for rabbits of all groups on 1d before operation, 1d, 4d, 1 week, 2 weeks,3weeks and 4 weeks after operation. Serum was separated. Enzyme linked immunosorbent assay was used for detection concentration of IL-17 family members(IL-17A-F). Results Concentration of IL-17 A,IL-27(IL-17B/C/D),IL-17E(IL-25),IL-17 F for rabbits after allogeneic skin grafting operation was higher than that before operation, concentration of IL-17 A,IL-27(IL-17B/C/D),IL-17E(IL-25),IL-17 F reached to the peak value on 2 weeks after operation, then concentration decreased. rhIL-10 could inhibit secretion of IL-17 A,IL-27 and IL-17 F. inhibiting effect was strengthened with the increase of rhIL-10 dose, rhIL-10 could promote secretion of IL-25,no obvious dose-effect relationship was found. Conclusion Serum IL-17 family level of rabbit treated with allogeneic skin grafting after injection of recombinational human IL-10 was obviously improved,inhibiting effect of r

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期刊信息
  • 《中国美容医学》
  • 主管单位:教育部
  • 主办单位:西安交通大学 第四军医大学
  • 主编:朱宏亮
  • 地址:西安市新科路1号东兴科技大厦12层
  • 邮编:710043
  • 邮箱:zgmryx@163.com yishi010@163.com
  • 电话:029-82218513 82251091-8833
  • 国际标准刊号:ISSN:1008-6455
  • 国内统一刊号:ISSN:61-1347/R
  • 邮发代号:52-27
  • 获奖情况:
  • 中国科技论文统计源期刊,中国学术期刊综合评价数据库来源期刊
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  • 被引量:28344