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中文摘要：
目的设计并验证适合Beckman AU5811全自动生化分析仪试剂针和搅拌棒的交叉污染分析方法,找到产生的原因并制定有效的污染避免措施。方法以反应盘内圈项目（ALT、TBIL、GGT、ALB、TBA、LDH1、CK、Cr、GLU、CO2、TC、HDL-C、APOA1、Ca、Fe、Cu、HCY、AFU、PA）作为实验项目,按设计的检测顺序进行交叉污染实验,各项目间的污染率以不超过95%~105%为判断标准。结果内圈项目中TC、HCL-C对TBA分别存在试剂针和搅拌棒的污染,Ca对Cr存在搅拌棒的污染,Cu对Fe存在试剂针的污染,GGT、ALB、LDH1对CO2都有搅拌棒的污染,TC、TBIL对Cu也存在搅拌棒的污染。结论采用该方法能有效快速地对Beckman AU5811生化分析仪上的所有项目进行交叉污染实验,确定交叉污染产生的关联项目并采取污染避免措施减少交叉污染,保证检验结果的准确。
英文摘要：
Objective To design and verify an analytical method for the cross contamination of Beckman AU5811 au- tomatic biochemical analyzer＇s reagent needle and stir bar and find the reasons, and make effective measures to avoid contamina- tion. Methods Use the inner ring items （ALT, TBIL, GGT, ALB, TBA, LDH1, CK, Cr, GLU, CO2, TC, HDL-C, APOAI , Ca, Fe, Cu, HCY, AFU, PA ） as the experiment objects and do the cross contamination experiment according to the test order. The standard for the cross contamination level is limited to 95-105% among all items. Results TC and HCL-C were found to have contaminated TBA both in reagent needle and stir bar model, Ca contaminated Cr in the stir bar model; Cu contaminated Fe in the reagent needle model; GGT, ALB and LDH1 contaminated CO2 in stir bar model; TC and TBIL contami- nated Cu in the stir bar model. Conclusion The method used in this experiment can effectively identify and analyze the cross contamination of all the items in Beckman AU5811 automatic biochemical analyzer. In addition, it can help to adopt correspond- ing measures to reduce cross contamination.
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