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中文摘要：
以厦门树白蚁（Glyptotennes Xiamenensis Li et Huang）为原料，经PBS缓冲液（pH6．7）抽提、硫酸铵分级分离获得纤维素酶粗提物（简称EGase），经DEAE-Sepharose Fast Flow阴离子交换柱层析、Shephadex G-100凝胶过滤柱层析等纯化，得到电泳单一纯的酶制剂，比活力为458．7U／mg．研究了金属离子对该酶活力的影响：结果表明，正-价金属离子Li^＋、Na^＋、K^＋对酶活力没有影响，Ca^2＋、Zn^2＋和Mn^2＋有激活作用，重金属离子Cu^2＋、Pb^2＋和Hg^2＋有抑制作用，表现为不可逆抑制．
英文摘要：
An endo-1,4-β-D-glucanase （EC#3.2.1.4）was purified from the termite Glyptotennes Xiamenensis Li et Huang by PBS buffer （ pH 6.7 ） extraction, ammonium sulfate precipitation, the ion-exchange chromatography on DEAE-Sepharose Fast Flow and the gel filtration through Sephadex G-100. The final preparation was homogeneous and electrophoretic pure with the specific activity of 458.7 U/mg. The effects of several metal ions on the enzyme activity were studied. Li^＋, Na^＋, K^＋ had no effects on the enzyme activity. The enzyme activity was stimulated by Ca^2＋ , Zn^2＋ and Mn^2＋ but inhibited by Cu^2＋ , Pb^2＋ , Hg^2＋ following the irreversible mechanism.
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