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中文摘要：
对实验室筛选的产精氨酸脱亚胺酶的假单胞菌（Pseudomonassp.）进行了固定化,以及对固定化细胞转化L-精氨酸生产L-瓜氨酸进行了研究。比较4种不同固定化方法,确定卡拉胶包埋结合戊二醛后处理为假单胞菌的细胞固定化方法。固定化反应的最适pH值为6.5,细胞固定化后细胞精氨酸脱亚胺酶的热稳定性增加。在50 mm×240 mm固定填充床反应器中,底物浓度为0.5 mol/L L-精氨酸盐酸盐,pH值6.5,温度37℃,稀释速率为0.147/h的条件下,连续运转54d,固定化细胞对底物的摩尔转化率在95%以上,平均生产强度0.010 8 g/（h.g）（每单位质量的固定化细胞每小时生产的瓜氨酸质量）。转化液经732阳离子树脂吸附,氨水洗脱,及浓缩、结晶,得到纯度为99%的L-瓜氨酸晶体,提取总收率约为87%。
英文摘要：
Preparation of L-citrulline from L-arginine by the immobilization of Pseudomonas sp, which was isolated by our laboratory, was studied in this manuscript. Entrapment with L- carrageenan followed by glutaraldehyde treatment was the best among four of immobilization methods. The optimal pH for conversion reaction with immobilized cells was 6.5. The thermostability of L-arginine deiminase was improved after immobilization. Using a column （50）〈 240） packed with the immobilized cells and 0.5mol/L L-arginine hydrochloride （pH6.5） at dilution rate of 0. 147/h and 37 ℃, the conversion was continuously operated for 54 days. The mol conversion yields were above 95%, and the average productivity of the immobilized cells was 0.0108 g/（h. g）. Then the effluent was adsorbed by resin 732, eluted with 5% NH3 · H2O,and concentrated by crystallization. Finally the L-citulline crystal with purity of 99% was obtained. The recovery rate of entire extraction process was about 87 %.
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