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中文摘要：
从作者所在实验室保存的3株白腐真菌中筛选到一株液态发酵产漆酶的密孔菌Pycnop-orus sp. SYBC-L1,以漆酶活力为指标,采用正交试验法,优化了Pycnoporussp.SYBC-L1分泌漆酶的培养基：麸皮水煮液60 g/L,葡萄糖60 g/L,豆粕粉15 g/L,CuSO4.5H2O 1.0 mmol/L;培养条件：初始pH 3.0,装液量50 mL/250 mL,30℃、200 r/min培养13 d,漆酶活力达24.95 U/mL,为优化前的36.16倍。
英文摘要：
In this study, the nutritional and environmental conditions of a laccase produce strain, Pycnoporus sp. SYBC-L1, were investigated and optimized though single factor experiments and orthogonal experiments. The optimum conditions described as follows： wheat bran cooking liquor 60 g/L, glucose 60 g/L, soybean powder 15 g/L and CuSO4 · 5H2O 1.0 mmol/L, pH 3.0, temperature 30℃, and culture volumn 50 mL/250 mL,respectively. With those optimum conditions, the laccase activity reached at 24.95 U/mL after 13 days culture.
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