欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
从土壤中筛选到1株产α-葡萄糖苷酶（EC3．2．1．20，α-glucosidase）的菌株WPD2，通过对其进行形态学和18SrDNA序列分析及系统发育研究，该菌株初步鉴定为黑曲霉（Aspergillus niger EU366904），该菌经产酶、转化反应后葡萄糖基抗坏血酸产量达到370mg／L左右。摇瓶实验确定了优化后的产酶条件为：培养温度34℃，培养时间60h，以110g／L的麦芽糖为碳源、10g／L的（NH4）2SO4为氮源，优化后葡萄糖基抗坏血酸产量达到410mg／L左右。
英文摘要：
An α-glucosidase-producing strain WPD2 was isolated from the soil, and was identified as As- pergillus niger based on morphology and phylogenetic analysis of 18S rDNA sequence. The content of ascorbic acid glucoside came to 370mg/L after biotransformation. The conditions for enzyme formation of strain WPD2 following the optimization phase were maltose 110g/L , ammonium sulphate 10g/L, culture time 60h,temperature 34℃ and the content of ascorbic acid glucoside reached 410mg/L.
同期刊论文项目
同项目期刊论文
期刊信息
位置：