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中文摘要：
从93株酵母、霉菌和细菌中筛选出能不对称还原2-辛酮的菌株.经过筛选获得一株博伊丁假丝酵母Candida boidinii CECT 10035.该菌种在底物浓度为78 mmol·L^-1时,能转化得到S-2-辛醇,2-辛酮转化率为32.6%,e.e.值达到95.6%.同时,还筛选出一株酒明串珠菌Oenococcus oeni CECT 4730,可以转化得到R-2-辛醇,当底物浓度为78 mmol·L^-1时,2-辛酮转化率为99.8%,e.e.值为97.8%.
英文摘要：
Asymmetric reduction of 2-octanone by yeast, mold and bacteria in aqueous-organic solvents biphasic systems were investigated. Candida boidinii CECT 10035 catalyzed the reduction of 2-octanoneto S-2-octanol with conversion of 32.6% and optical purity of 95.6%. Different from the yeast strain,Oenococcus oeni CECT 4730 catalyzed the reduction of 2-oetanone to R-2-octanol with higher activity and optical purity. When the concentration of 2-octanone was 78 mmol·L^-1, the conversion and enantiomeric excess were 99.8% and 97.8%.
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