甘油为微生物可利用的理想碳源,从自然界筛选出21株以甘油为唯一碳源产二羟丙酮(DHA)的菌株,经初步发酵测定发酵液中DHA含量,其中菌株6-8DHA产量最高达6.4g/L。对其进行常规生理生化鉴定实验,并结合16S rDNA基因分析,比对结果表明,菌株6-8与Acinetobacter sp.相似性最高,达99.7%,在细菌分类学上属于假单胞茵目莫拉茵科不动杆菌属。将其命名为Acinetobactersp.6-8。
More than 20 strains capable of producing dihydroxyacetone from glycerol were isolated from 4 different natural environment samples by using two detection methods, The strain 6-8 which could grow on medium containing glycerol as sole carbon source had a higher converting capability. Under a better culture, the highest DHA production of the strain 6-8 reached 6.4 g/L. In addition to general morphological and biochemical characteristics, the strain 6-8 was identified by 16S rDNA sequence and systematic analysis. The results showed that 16S rDNA sequence of the strain 6-8 had similarity of 99.7% with Acinetobacter sp. suggesting that the strain 6-8 is one of subspecies of Acinetobacter sp.