用二乙酰一肟-氨基硫脲比色法测定酶转化液中的L-瓜氨酸含量,并研究了测定波长、氨基硫脲用量、铁离子等因素的影响。线性范围为0.1~20mg/L(r=0.999),回收率99.8%。
A diacetylmonoxime-thiosemicarbazide colorimetry was established for determination of L-citrulline in enzymatic conversion solution. The influence factors including absorption wavelength, thiosemicarbazide concentration and Fe^3+ were investigated. The linearity range was 0.1 - 20mg/L (r = 0.999), and the average recovery was 99.8 %.