酚类化合物是大麦中主要的抗氧化物质之一,直接影响到麦芽、麦汁和啤酒的品质。研究了体积分数100%的甲醇、体积分数80%的甲醇、体积分数80%的乙醇、体积分数80%的丙酮和水等5种不同提取溶剂对大麦游离多酚和结合多酚提取率的影响,以及不同溶剂提取物清除DPPH自由基能力的差异。结果表明:提取溶剂对大麦多酚的提取效率有显著影响,不同溶剂提取物清除DPPH自由基的能力有显著性差异;其中以80%丙酮溶液为提取溶剂时,所得提取物的游离多酚质量分数及其对DPPH自由基清除率最高,可作为大麦抗氧化物质的提取溶剂;不同品种大麦间游离总多酚质量分数及其对DPPH自由基清除率存在显著差异;结合Folin-Ciocalteu法和DPPH自由基清除率法分别测定大麦总多酚质量分数和抗氧化力,可初步评价大麦原料的抗氧化特性。
Phenolic compounds are the most important antioxidant in barley, which influence the qualities of malt, wort and finished beer directly. Effects of different extraction solvents (100% methanol, 80% methanol, 80% ethanol, 80% acetone and water) on the total polyphenol yield and antioxidant activity of barley were studied. The results showed that the extracting solvent significantly influenced the total free and bound polyphenol yield and antioxidant activity from barley extracts. 80% acetone is a selected solvent for extracting phenolic antioxidant from barley. Differences in total phenolics content and antioxidant activity of different barley varieties were observed. The Folin-Ciocalteu method for total phenolics content and the free radical DPPH scavenging method for antioxidant activity could be used as estimation of barley antioxidant activity.