欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
对重组荧光素酶大肠杆菌菌株M15／pQE30-luc进行了表达条件的优化研究。单因素结果表明：在初始pH值7．0，装液量为20％，2％的接种量，终浓度为0．5mmol／L的IPTG，添加10—30mmol／L的Mg^2＋，摇床转速为200r／min，37℃诱导3．5h酶的表达量最高。正交试验结果表明：初始pH值为7．0，添加40mmol／LMg^2=，接种量2％，装液量为20％时表达量最高，比酶活达1．63×10^8RFU／mg蛋白。
英文摘要：
The luc gene was subcloned into expression vector pQE30 and its product LUC was overexpressed in Escherichia coli M15. The optimum fermentation condition was： pH 7.0, the amount of medium 20% , inoculum size 2%, the final concentration of IPTG 0.5 mmoL/L, 10 - 30 mmol/L Mg^2 ＋ , the rotating speed 200 r/min, inducing 3.5h at 37℃. After orthogonal experimental design, the result showed that the initial pH7.0, 40mmol/L Mg^2＋ , 2% inoculation amount and the 20% medium amount had the best expression of LUC, and the specific activity was 1.63 × 10^8 RFU/mg protein.
同期刊论文项目
同项目期刊论文
Comparative Characterization of Genes Encoding Glycerol 3-phosphate Dehydrogenase From Candida glyce
Cloning and characterization of a NAD(+)-dependent glycerol-3-phosphate dehydrogenase gene from Cand
Transformation of Industrialized Strain Candida glycerinogenes with Resistant Gene zeocin via Agroba
期刊信息
位置：