对北沙参内生真菌类群组成及多样性进行分析。结果表明,从北沙参植株中分离到内生真菌137株,利用ITS-r DNA序列分子鉴定法将北沙参生真菌鉴定为21个分类单元,分布在6纲8目11科21属。河北安国北沙参内生真菌归于10个属,内生真菌的多样性指数H=1.08、D=0.90,优势菌群为链格孢属(Alternaria)真菌,占总菌株数的29.93%;山东烟台北沙参内生真菌归于17个属,内生真菌的多样性指数H=1.31、D=0.94,以刺盘孢属(Colletotrichum)和链格孢属(Alternaria)真菌为优势菌群,分别占总菌株数的19.71%和13.87%,两地北沙参内生真菌种类的相似性系数Cs=0.57。由此可见,两地北沙参内生真菌组成具有一定的相似性,山东烟台北沙参内生真菌多样性高于河北安国北沙参。
The endophytic fungi were isolated from Glehnia littoralis. The molecular identification of ITSr DNA was applied to analyze fungal taxa,and fungal diversity was explored. The results showed that 137 fungal strains were isolated from G. littoralis. These fungi were assigned to 21 taxa,containing 6 classes,8 orders,11 families and 21 generas. Endophytic fungal groups in G. littoralis of Anguo were attributed to 12 generas. ShannonWiener index(H)and Simpson’s index(D)were 1.08 and 0.90 respectively. Alternaria(29.93%)was the major fungal group. Endophytic fungal groups in G. littoralis of Yantai were attributed to 17 generas. The diversity index were 1.31(H)and 0.94(D)respectively. Colletotrichum(19.71%)and Alternaria(13.87%)belonged to the core groups. Thus,the diversity of endophytic fungi in Yantai G. littoralis was higher than that of Anguo G. littoralis. Moreover,there was a medium similarity(Cs=0.57)of endophytic fungal composition in G. littoralisbetween Anguo and Yantai.