以羊角椒不育系O-6、O-9为试材,采用分期播种方法,在盛花期分别对两份雄性不育试材5个发育时期(造孢细胞时期、花粉母细胞时期、四分体时期、单核小孢子时期、成熟花粉粒时期)花药的可溶性糖、可溶性蛋白、游离脯氨酸含量进行测定。结果表明:随着播种时间的后移,同生育期的花药中可溶性糖、可溶性蛋白、游离脯氨酸含量均呈下降趋势,在造孢细胞时期含量最高。但同一播期的不同发育期的花蕾中可溶性糖含量是先上升后下降,在四分体时期含量最高,O-6为2.17mg/g·FW,O-9为1.23 mg/g·FW。
The hot pepper male sterile lines O-6 and O-9 was used as experimental materials and they were sowedat interval. In full flowering stage, changes of the content of soluble sugar, soluble protein and proline were deter-mined at 5 development stage, i.e., the stage of sporogenous cells, the stage of microspore mother cells, the stage oftetrads, the stage of monoclear microspores and the stage of pollen grains.The results showed that when sowing datewas postponed, the content of soluble sugar, soluble protein and proline in anthers of the same growing period de-creased. The highest content was at the stage of sporogenous cells. But the content of soluble sugar in flowers at thesame sowing date increased firstly and decreased at last and the highest content was at the stage of tetrads, O-6 was2.17 mg/g·FW, O-9 was 1.23 mg/g·FW.