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Characterization of Bacterial Strains Isolated from a Novel Seawater-based Retting Treatment of Hemp
  • ISSN号:1000-7555
  • 期刊名称:《高分子材料科学与工程》
  • 时间:0
  • 分类:S852.659.5[农业科学—基础兽医学;农业科学—兽医学;农业科学—畜牧兽医] TQ458[化学工程—农药化工]
  • 作者机构:[1]College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China, [2]Key Laboratory of Advanced Textile Material and Manufacturing Technology, Ministry of Education, College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, China, [3]The Research Center of China-hemp Materials, The Quartermaster Research Institute of the General Logistic Department of CPLA , Beijing 100088 , China
  • 相关基金:Foundation items, Program for Changjiang Scholars and Innovative Research Team in University, China( No. IRT0654 ) ; Major State Basic Research Development Program, China ( No. 2008 CB617506 ); Analysis and Test Foundation of Zhejiang Province, China (No. 2007F70040)
中文摘要:

Cultivable bacteria were isolated from seawater-based retting treatment of hemp, in which three of purified strains (SW- 1, SW- 2, and S-SW1) produced relatively high levels of pectinase activities, and also produced mannanases and xylanases. PCR-based entebacterial repetitive intergenic consensus primers (ERIC-PCR) were employed for fingerprinting DNA of the bacterial strains. The ERIC-PCR fingerprints of stains SW- 1, SW- 2, and S-SW1 were found to be different, and should be further identified for each isolate. Strains SW- 1 and SW- 2 were identified as Stenotrophomnas maltophilia, while strain S-SW1 was assigned to Ochrobactrum anthropi by BIOLOG system. These two species represented rhizosphere bacterial genera, and possibly were introduced by the hemp plants. These organisms seemed potentially capable of producing pectinase and hemicellulase, and thus effectively degrading the gum substances in the seawater retting. This research could be helpful for improving a novel seawater-based retting treatment of hemp.更多还原

英文摘要:

Cultivable bacteria were isolated from seawater-based retting treatment of hemp, in which three of purified strains (SW- 1, SW- 2, and S-SW1) produced relatively high levels of pectinase activities, and also produced mannanases and xylanases. PCR-based entebacterial repetitive intergenic consensus primers (ERIC-PCR) were employed for fingerprinting DNA of the bacterial strains. The ERIC-PCR fingerprints of stains SW- 1, SW- 2, and S-SW1 were found to be different, and should be further identified for each isolate. Strains SW- 1 and SW- 2 were identified as Stenotrophomnas maltophilia, while strain S-SW1 was assigned to Ochrobactrum anthropi by BIOLOG system. These two species represented rhizosphere bacterial genera, and possibly were introduced by the hemp plants. These organisms seemed potentially capable of producing pectinase and hemicellulase, and thus effectively degrading the gum substances in the seawater retting. This research could be helpful for improving a novel seawater-based retting treatment of hemp.

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期刊信息
  • 《高分子材料科学与工程》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国教育部
  • 主办单位:中国石油化工股份有限公司科技开发部 国家自然科学基金委员会化学科学部 高分子材料工程国家重点实验室 四川大学高分子研究所
  • 主编:王琪
  • 地址:四川大学(西区)高分子研究所
  • 邮编:610065
  • 邮箱:GFZCLBJB@163.com
  • 电话:028-85401653
  • 国际标准刊号:ISSN:1000-7555
  • 国内统一刊号:ISSN:51-1293/O6
  • 邮发代号:62-67
  • 获奖情况:
  • 教育部1999年全国优秀期刊二等奖,1999年四川省优秀科技期刊一等奖,2000年四川省优秀期刊二等奖,中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),荷兰文摘与引文数据库,美国工程索引,美国剑桥科学文摘,英国高分子图书馆,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:30425