对我国主要花生种植区的170个花生品种的蛋白质组成进行SDS-PAGE电泳分析。结果表明:不同花生品种的蛋白组分和亚基相对含量在种质材料间存在差异。各品种均含有花生球蛋白和伴花生球蛋白,花生球蛋白与伴花生球蛋白比值变化范围介于0.80~1.68之间,变异系数为15.23%,大于10%,说明不同花生品种蛋白组成比例存在较大的变异性。各品种亚基变异显著,其中,花生球蛋白的35.5kD亚基差异最显著,变异系数达55.07%,双纪2号等35个品种缺失35.5kD亚基条带,占所测试品种的20.59%。相关性分析表明,花生球蛋白与伴花生球蛋白之间呈极显著负相关(r=-0.998)。
The composition of peanut protein from 170 different cultivars in China was analyzed by SDS-PAGE as well as the relative content of major fractions.There were significant variations in protein composition and the relative content of subunits among these peanut cultivars.Both arachin and conarachin were found in each cultivar,with a ratio between 0.80 and 1.68,and a coefficient of variation(CV) of 15.23%.This CV larger than 10% suggests great differences in protein composition among different peanut cultivars.In addition,significant variations were also observed in the relative content of subunits;in arachin,the 35.5 kD subunit presented the largest CV of 55.07%,which,however,was absent from 20.59%(35/170,such as ShuangJi 35) of all the tested cultivars.Moreover there was an extremely significantly negative correlation between arachin and conarachin(r =-0.998).