黄曲霉毒素主要是由黄曲霉和寄生曲霉产生的一类次级代谢产物,经常污染粮油原料及其制品,具有强致癌性.介绍了黄曲霉毒素毒性及一些国家和地区的残留限量标准;针对不同基质间组分存在较大差异,概述了液液萃取、超临界流体萃取、固相萃取及免疫亲和柱净化等样品前处理方法;同时对薄层色谱、高效液相色谱、酶联免疫吸附等测定黄曲霉毒素的方法、原理、应用及特点进行了综述;最后分析了黄曲霉毒素检测技术的发展趋势.
Aflatoxins (AFs) are seriously carcinogenic and secondary metabolites mainly produced by Aspergillus flavus and Aspergillus parasiticus,often pollute grains materials and its products.The toxicity of AFs and residual limits standards in different countries and regions were introduced.According to the differences for matrices,the methods of sample pretreatment including liquid-liquid extraction,supercritical fluid extraction,solid phase extraction and immunoaffinity were summarized.Meantime,the determination methods,principles,applications and features of AFs such as TLC,HPLC,and ELISA were introduced.Finally,the development trend of detection technology for AFs was analyzed.