研究了傅立叶变换红外(FT-IR)光谱法快速检测籼米淀粉回生,发现FT-IR光谱中一些特征振动模式的相对强度(与761.76 cm^-1处峰高的比值)会随淀粉回生程度的增加而降低.振动模式包括:3 415 cm^-1附近的O—H伸缩振动、2 927.46 cm^-1处的CH2伸缩振动、1 155.17 cm^-1处C—O和C—C伸缩振动、1 081.89 cm^-1处的C—O—H弯曲振动、1 043.32,1 020.18和998.96 cm^-1处未确定的振动模式、609.41和578.55 cm^-1处的吡喃环骨架振动模式.将以上这些特征振动模式的相对强度倒数与DSC回生焓值进行相关性分析可知:1 081.89,1 043.32,1 020.18,609.41和578.55 cm^-1处吸收峰的相关系数均能达到0.9以上,因此可将这5种FT-IR振动模式的相对强度变化作为定量指示淀粉回生程度的多重指标.
The rapid detecting of the retrogradation for long-shaped rice starch by FT-IR was investigated.The results show that the relative intensity of characteristic peaks which is defined as the peak height ratio to the reference peak of 761.76 cm^-1 is decreased with the retrogradation increase.These characte-ristic peaks include O—H stretching at 3 415 cm^-1,C—H stretching at 2 927.46 cm^-1,C—O and C—C stretching at 1 155.17 cm^-1,C—O—H bending at 1 081.89 cm^-1,undetermined modes at 1 043.32,1 020.18 and 998.96 cm^-1,skeletal modes of pyranose ring at 609.41 and 578.55 cm^-1.According to the correlation analysis between retrogradation enthalpy of DSC and relative intensity reciprocal of above vibration modes,the FT-IR peaks at 1 081.89,1 043.32,1 020.18,609.41 and 578.55 cm^-1 have correlation coefficients all above 0.9.The relative intensity change of the five vibration modes listed above can be used as multi-index to quantify the retrogradation degree.