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盐酸小檗碱对体外培养人牙周膜细胞生物活性的影响

ISSN号：1001-3733
期刊名称：《实用口腔医学杂志》
时间：0
分类：R781.42[医药卫生—口腔医学;医药卫生—临床医学]
作者机构：[1]兰州大学口腔医学院,730000, [2]兰州大学中西医结合研究所
相关基金：甘肃省科技攻关项目（编号：2GS064-A43-020-34）;兰州大学医学基金（编号：2006-23）

关键词：盐酸小檗碱, 人牙周膜细胞, 增殖, 碱性磷酸酶, Berberine hydrochloride , Human periodontal ligament cells , Proliferative , Alkaline phosphatase

中文摘要：

目的：探讨盐酸小檗碱对原代培养的人牙周膜细胞（PDLC）生物活性的影响。方法：采用细胞培养技术、噻唑蓝（MTT）比色法、考马斯亮蓝法、酶动力学方法观察盐酸小檗碱对PDLC增殖活性、蛋白合成及碱性磷酸酶（ALP）活性的影响。结果：①与对照组比较，作用24h，盐酸小檗碱（0．005—0．030g／L）能明显增强人牙周膜细胞增殖能力（P〈0．05）；作用48h，盐酸小檗碱（0．005—0．020g／L）能明显增强人牙周膜细胞增殖能力（P〈0．05）；作用72h，盐酸小檗碱（0．010—0．020g／L）能明显增强人牙周膜细胞增殖能力（P〈0．05）。②盐酸小檗碱（0．005—0．020g／L）细胞培养液中蛋白总含量均明显高于对照组（P〈0．05）。③盐酸小檗碱（10—20g／L）细胞培养液中ALP活性均明显高于对照组（P〈0．05）。结论：盐酸小檗碱在0．010—0．020g／L浓度范围内有促进PDLC的增殖及生物合成作用，能增强牙周膜细胞ALP活性。

英文摘要：

Objective： To explore the effects of berberine hydroehloride on human periodontal ligament cells （HPDLCs） cultured in vitro. Methods：Cell proliferation activity was assayed by using MTT method. The total protein was detected by Coumassie Bright Blue method and the alkaline phosphatase （ALP） activities were measured by spectrophotometric assay. Results：（1）Comparing with the control group, the proliferative ability of PDLCs in berberine hydrochloride （0. 005,0. 010,0,020,0. 030 g/L） groups at 24 h and （0. 005,0. 010,0. 020 g/L） groups at 48 h and （0. 010,0.020 g/L）groups at 72 h were considerably increased （ P 〈 0.05 ）. （2） Comparing with the control group, the total amount of protein of PDLCs in berberine hydrochloride （0.005,0.010,0.020 g/L）groups were considerably increased（P 〈 0.05）. （3） Comparing with the control group, ALP activity of PDLCs in berberine hydrochloride （0.010,0.020 g/L） groups at 72 h were significantly enhanced （P 〈 0.05 ）. Conclusion：The results suggest that berberine hydroehloride （0.010,0.020 g/L） could promote proliferation ability, protein biosynthesis and ALP activity of PDLCs. It may play a role in periodontal tissues regeneration.

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