采用蛋白酶水解鲜鸡蛋蛋黄,分离得到卵黄非磷肽,测定了卵黄非磷肽的分子组成及其抑菌活性.研究结果表明:卵黄非磷肽的相对分子质量分布在158~1493之间,其中酸性氨基酸成分较多,达到23.6%.卵黄非磷肽对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌具有抑制作用,在pH4.0~5.0显示稳定的抑菌活性,121℃和紫外线照射处理对其活性无明显影响,常温长时间(72h)放置其抑菌活性保持稳定.
Egg yolk non-phosphopetide was separated from egg yolk protease hydrolyzes. The molecular composition and antibacterial activity of egg yolk non-phosphopetide were determined. The results showed that the relative molecular weight of egg yolk non-phosphopeptide was in the range of 158 - 1 493, and it consists mainly of acidic amino acid, about 23.6%. The egg yolk non-phosphopeptide had antibacterial activity against Escherichia coli,Staphylococcus aureus Rosenbach and Bacillus subtilis Lonn that was stable between pH 4 - 5 and under strong heating condition ( 121 ℃ ) , and also stable preserved after treatment with ultraviolet light and a long-time (72 h) keeping at room temperature.