中文摘要：

目的探索凝血块中提取淋巴细胞的方法 ,分析抗凝血与凝血块中T细胞的活性。方法剥离凝血块并转移至过滤网上,生理盐水湿润并在滤网上轻柔研磨凝血块,收集滤过细胞。淋巴细胞分层液分别分离抗凝血与等量血凝块研磨过滤细胞,获取PBMC,流式细胞术检测并比较T细胞表面CD分子表达情况。T细胞增殖试验检测并比较抗凝血与凝血块放置不同时间（4,8,12和24 h）所得PBMC中T细胞的增殖活性。结果 2种血样所得T细胞表面CD标志的百分率无差别（P〉0.05）。血液凝固12 h内不影响所得T细胞的活性（各组SI比较,P〉0.05）。结论获得了凝血块提取淋巴细胞的方法,并证明此方法不影响凝血12 h内所得T细胞的活性。

英文摘要：

This study designed to explore the method of separating lymphocyte from blood clot, and analyze the activity of T lymphocyte in anticoagulated blood and blood clot. The blood clot was avulsed and soaked with saline, grinded softly on filter. After collecting the filtered cell from blood clots, the peripheral blood mononuclear cell（PBMC） from the blood clot（filtered cell） and equivalent anticoagulated blood were separated through ficoll separation assay. Furthermore, CD molecule expression on T cell surface of the two groups was compared by flow cytometry; the proliferation activity of T cell from anticoagulated blood sample and blood clot samples of different time points（congealed for 4 h/8 h/12 h/24 h） were detected and compared. Results showed that there was no difference in the percentage of CD markers of T cell between the two groups（P 〉0.05）; the activity of T cell in blood clot did not change within 12 h（P 〉0.05）. Thus we concluded that the method can effectively separate T cell from blood clots, and the activity of obtained T cell was as same as that of anticoagulated blood within 12 hours.