中文摘要：

本试验在产犊后立即无菌采集32头奶牛血液,其中17头为胎衣正常排出奶牛,15头为胎衣不下奶牛。胎衣正常排出奶牛在胎衣排出后立即采集胎盘,胎衣不下奶牛在确诊后再次静脉采血并采集其胎盘;用酶联免疫吸附测定法对胎衣不下奶牛和胎衣正常排出奶牛血清和胎盘中的激素含量进行检测,并对检测结果进行分析。结果表明,胎衣不下奶牛血清和胎盘中孕酮、纤维蛋白溶酶原和前列腺素（PGF2α）的含量与胎衣正常排出组相比差异显著降低（P〈0.05）,而雌二醇含量则显著升高（P〈0.05）。本试验通过对胎衣不下和胎衣正常排出奶牛血清和胎盘中激素的含量进行检测并比较,结果发现奶牛胎衣不下的发生与血清和胎盘组织中的孕酮、雌二醇、纤维蛋白溶酶原和前列腺素（PGF2α）的含量变化有密切相关的联系,表明奶牛胎衣不下的发生可能与机体内激素分泌紊乱具有很大的关联。

英文摘要：

In order to study the change characteristics of hormone in serum and placenta of retained placenta cows and establish the prevention and treatment of retained placenta, which can elucidate the pathogenesis of the disease. this study aims to analysis the serum and placental hormone of retained placenta cows based on enzyme-linked immunosorbent method. Thirty-two serums were collected after calving, of which seventeen were cows without retained fetal membranes and fifteen were cows with retained fetal membranes. Placenta were collected after serum were collected in cows without retained fetal membranes. The venous blood and the placenta were collected after the cows with retained fetal membranes were diagnosed. The enzyme-linked immune adsorbent were used to test the hormone levels in the serum and placenta of cows with retained fetal membranes and cows without retained fetal membranes, and the testing results were analyzed. The results showed that the content of of progesterone, fibrinogen, and the prostaglandin in the serum and placenta of cows with retained fetal membranes compared with the cows without retained fetal membranes group differences significantly reduced （P〈0.05）, but estradiol levels were significantly increased （P〈0.05）. The hormone content in serum and placenta of cows with retained fetal membranes and cows without retained fetal membranes were tested in this experiment, which found that the morbidity of retained fetal membranes were associated with the the content changes of progesterone, estradiol ,fibrinogen and prostaglandin in serum and placental tissue, which indicates that retained fetal membranes can happen may associated with hormone secretion disorder within the body.