中文摘要：

为了探索连梅提取液对湿热泻痢仔猪的抗氧化功能、胃肠调节酶及十二指肠凋亡基因表达的影响,试验采用了人工模拟高湿高热的环境,给仔猪灌服大肠杆菌辅助致使泻痢,选择泻痢仔猪分为模型组、阳性药物组和连梅提取液高、中、低剂量组,以正常环境下正常饲喂的仔猪作为对照,通过检测仔猪血清抗氧化酶、胃肠调节酶、肝ATP酶以及十二指肠凋亡基因Caspase-3、-8、-9 mRNA转录变化以分析连梅提取液治疗泻痢仔猪的作用机制。结果显示,湿热泻痢仔猪经连梅提取液进行治疗后,血清T-SOD、GSH-Px活力升高,LDH、MDA含量减少,与模型组相比较差异极显著（P〈0.01）;乙酰胆碱酯酶、淀粉酶、肝Na＋-K＋-ATPase和Ca2＋-Mg2＋-ATPase活力较模型组显著增强（P〈0.05）;T3、T4和GH分泌量显著升高（P〈0.05）,GC分泌量显著降低（P〈0.05）;同时各药物组仔猪十二指肠凋亡基因得到了不同程度的恢复,Caspase-8 mRNA表达量只有中剂量组较模型组极显著降低至2.62倍（P〈0.01）;Caspase-3mRNA表达中,高、中剂量组分别降至正常组的5.82、3.29倍,较模型组差异显著（P〈0.05）或极显著（P〈0.01）;Caspase-9mRNA表达量均显著降低（P〈0.05）,且降低量与药物浓度成正比。由此可知,连梅提取液可以通过增强湿热泻痢仔猪抗氧化损伤能力,改善胃肠道的消化吸收,促进生长激素的分泌和降低凋亡基因的表达而达到治疗仔猪湿热泻痢。

英文摘要：

In order to explore the effect of Lianmei extract on antioxidant index,gastrointestinal regulatory enzymes and duodenal apoptosis gene expression of heat and humidity diarrhea swine.Established heat and humidity environment artificially,and the swine fed E.coli to induce heat and humidity diarrhea model.The diarrhea swine were randomly divided into model group,positive drug group and Lianmei extract with high-,middle-and low dose groups,swine in the control group were fed at normal environment.Detected antioxidant enzymes,gastrointestinal regulatory enzymes,liver ATP enzymes vitality and duodenum apoptosis gene Caspase-3,-8,-9 mRNA expression changes for analysing the treatment mechanism of Lianmei extract.The results showed that the swine treated by Lianmei extract,The serum content of T-SOD and GSH-Px enzyme activity increased,LDH and MDA reduced,compared with model group,the difference was extreme-ly significant（P〈0.01）.Acetylcholinesterase,amylase,liver Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase vitality significantly increased（P〈0.05）.Growth hormone,thyroid hormone T3 secretion improved significantly（P〈0.05）,Glucocorticoids secretion decreased significantly（P〈0.05）.Meanwhile,duodenal apoptosis genes of swine recoveryed at different degree.Only the middle dose group Caspase-8 mRNA transcription decreased significantly to 2.62 times of that from the control（P〈0.01）.High-and middle dose group Caspase-3mRNA transcription were reduced to 5.82 and 3.29 times of that from the control,compared with the model group,the difference was significant（P〈0.05）or extremely significant（P〈0.01）.However,Caspase-9mRNA transcription was significantly reduced（P〈0.05）at high-,middle,low dose group and the amount of reduction was proportional to the concentration of the drug.The results indicated that Lianmei extract could achieve therapeutic heat and humidity diarrhea swine by enhancing diarrhea pigs anti-oxidative damage,improving gastrointestinal digestion and absorption capacity,pr