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中文摘要：
目的：建立黄白止痢颗粒的质量标准。方法：采用薄层色谱法对黄白止痢颗粒中黄连、大黄炭、白头翁进行定性鉴别,并采用高效液相色谱法测定盐酸小檗碱含量。Agilent Eclipse XDB-C18色谱柱（4.6 mm×250 mm,5μm）;流动相乙腈-0.3%磷酸水溶液（32∶68）,检测波长348 nm,柱温25℃,流速1.0 mL·min-1,进样量10μL。结果：在薄层鉴别中黄连、大黄炭、白头翁有良好的鉴别特征有效成分盐酸小檗碱含量在0.008 8～0.11 g·L-1具有良好的线性关系（r=0.999 1）,平均加样回收率为98.58%,RSD 1.05%。结论：使用薄层色谱法对黄连、大黄炭、白头翁进行定性鉴别,操作简便、重复性好,且阴性对照无干扰;盐酸小檗碱的含量测定方法简便、快速、准确,具有良好的重复性和回收率,可作为黄白止痢颗粒的质量控制方法。
英文摘要：
Objective：To establish the quality standard of Huangbaizhili Granules.Method：The Coptis chinensis,Rhubarb charcoal and Pulsatilla chinensis were identified by TLC and the content of berberine hydrochloride was detected by HPLC.The separation was carried out on an Agilent Eclipse XDB-C18 column（4.6 mm × 250 mm,5 μm）.The mobile phase consisted of acetonitrile-0.3% phosphoric acid（32：68）.The detective wavelength was set at 348 nm.The column temperature was kept at 25 ℃.The flow rate was 1.0 mL·min-1and the injection volume was 10 μL.Result：The C.chinensis,R.charcoal and P.chinensis could be identified by TLC and the calibration curve of berberine hydrochloride showed a good linearity in the range of 0.008 8-0.11 g·L-1（r =0.999 1）.The average recovery was 98.58% with RSD 1.05%.Conclusion：This method to identify three main ingredients in Huangbaizhili Granules by TLC is simple and has a great repeatability.The negative control sample has no interference.The method to determine the content of berberine hydrochlorid in Huangbaizhili Granules is rapid,accurate and the results have a good repeatability and recovery.The methods can be used for the quality of the Huangbaizhili Granules.
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