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中文摘要：
【目的】从患中华鳖出血性肠道坏死症病鳖体内分离病原菌并进行鉴定与药敏特性分析,为有效防控中华鳖出血性肠道坏死症提供参考依据。【方法】在无菌条件下分别从病鳖腮腺、肠道、肝脏、精巢及卵巢等病变器官中分离病原菌,然后进行人工回归感染试验、生理生化与分子鉴定及系统发育树分析,并采用K-B纸片扩散法进行药敏试验。【结果】从患中华鳖出血性肠道坏死症的病鳖体内分离出5株菌株JZ-01、JZ-02、JZ-03、JZ-04、JZ-05,人工回归感染试验结果表明这5株菌株对中华鳖的致死率均达100.00%。5株菌株均为革兰氏阴性菌,无芽孢,在普通营养琼脂培养基上生长呈灰白色、边缘整齐、半透明、湿润的菌落;通过梅里埃VITEK-2全自动微生物鉴定仪对5株菌株进行生理生化鉴定,证实均为摩氏摩根菌（Morganella morganii）,且5株菌株（GenBank登录号分别为KP054384、KP054385、KP054386、KP054387和KP054388）与摩氏摩根菌菌株（GenBank登录号JF508858.1）的亲缘关系最近。5株菌株对复方新诺明、多粘霉素B、诺氟沙星、强力霉素、丁胺卡那霉素、氟苯尼考等6种抗生素高度敏感。【结论】摩氏摩根菌能引起中华鳖出血性肠道坏死症,对中华鳖具有很强的致病性,生产中可选用复方新诺明、多粘霉素B、诺氟沙星、强力霉素、丁胺卡那霉素、氟苯尼考等药物进行防治。
英文摘要：
[Objective]The present experiment was conducted to isolate pathogenic bacteria from Trionyx sinensis suffering with hemorrhagic intestinal necrosis,identify them and determine their drug sensitivity,in order to provide references for effectively preventing and controlling hemorrhagic intestinal necrosis disease of T.sinensis.[Method]The pathogenic bacteria were isolated from parotid,liver,intestine,testis and ovary of diseased T.sinensis under aseptic conditions.The isolated bacteria were studied through artificial infection experiment,physiological,biochemical and molecular identification,and phylogenetic tree analysis.Then the drug sensitivity of pathogenic bacteria was determined using KB paper diffusion method.[Result]The results showed that,five strains of pathogens were isolated from T.sinensis suffering with hemorrhagic intestinal necrosis,which were named JZ-01,JZ-02,JZ-03,JZ-04 and JZ-05.The artificial recursive infection experiment showed that lethality rate of these isolates for T.sinensis was up to 100%.And the physiological and biochemical identification showed that they were Gram-negative bacteria without spore,and formed into gray semitransparent hygric colonies with neat edges on ordinary nutrient agar plate.And these strains were identified as Morganella morganii using BioMerieux VITEK-2 automated microbial identification system.Furthermore,the phylogenetic analysis results indicated that these bacteria（GenBank accession number：KP054384,KP054385,KP054386,KP054387,KP054388） had the closest genetic relationship with M.morganii（GenBank accession number：JF508858.1）.In addition,the drug sensitive test showed that these strains were highly sensitive to cotrimoxazole,polymyxin B,norfloxacin,doxycycline,amikacin and florfenicol.[Conclusion]M.morganii,has high pathogenicity for T.sinensis,can cause hemorrhagic intestinal necrosis.T.sinensis suffering with hemorrhagic intestinal necrosis could be treated by cotrimoxazole,polymyxin B,norfloxacin,doxycycline,amikacin,florfenicol.
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