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中文摘要：
本研究从自然发病的养殖大黄鱼（Pseudosciaena crocea）肝脏处分离得到1株优势菌9L2,经形态学观察和传统生理生化鉴定,显示该菌为发酵型革兰氏阴性短杆菌,有动力,氧化酶阳性,水解赖氨酸及色氨酸等。利用Biolog细菌鉴定方法进行种属鉴定,确定9L2为维氏气单胞菌（Aeromonas veronii）,相似性达100%。同时对其16S rDNA基因进行PCR扩增、测序和系统发育分析,结果表明该菌与气单胞菌属的菌株亲缘关系最近,与维氏气单胞菌（登录号为LMG13695）的16S rRNA基因序列相似性达99.79%。经人工腹腔注射感染吉富罗非鱼（Oreochromis niloticus）的试验结果表明,9L2对罗非鱼的半数致死剂量LD（50）为1.08×10～3CFU/g。24种抗生素药敏试验结果表明,该菌株对诺氟沙星、新生霉素、呋喃妥因、头孢曲松等13种药物高度敏感,对洛美沙星、卡那霉素等5种药物中度敏感,对利福平、青霉素等6种药物产生耐药性。20种中药体外抑菌试验结果表明,其中11种对致病菌体外抑菌效果较好,平均抑菌浓度范围为0.11—7.20mg/mL。
英文摘要：
A dominant bacterial strain,named 9L2,was isolated from the liver of diseased Pseudosciaena crocea and identified by biochemical characterization,Biolog identification,and 16 S rRNA gene sequence analysis.The bacteria was fermented,Gram-negative,motile,and oxidase-positive,could degrade lysine and tryptophan based on morphological,physiological,biochemical tests.The analysis of 16 S rRNA gene sequence show that the organism and the type strain of Aeromonas veronii（LMG13695） formed consistently a monophyletic clade with a distant sequence similarity of 99.79%.According to the results,the bacterium was identified as A.veronii.Moreover,lethal dose and 50% trials were carried out to demonstrate the virulence of 9L2 in Oreochromis niloticus when administered at 1.08×10~3CFU/g.Drug sensitivity test on 24 antimicrobial agents and 20 Chinese herbs showed that the strain were very sensitive to 13 agents（norfloxacin,novobiocin,nitrofurantoin,ceftriaxone,etc.）,while mild or not sensitive to the rest;of 20 Chinese herbs,11 herbs showed better antibacterial effect in vitro in average minimum inhibitory concentration ranging from 0.11 to 7.20mg/m L.
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