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斑点叉尾鮰病原中温和气单胞菌的分离鉴定及药敏特性分析

ISSN号：1005-3832
期刊名称：《水产学杂志》
时间：0
分类：S94[农业科学—水产养殖;农业科学—水产科学]
作者机构：[1]中国水产科学研究院长江水产研究所,武汉430223, [2]淡水水产健康养殖湖北省协同创新中心,武汉430223, [3]中国水产科学研究院、农业部水产品质量安全控制重点实验室,北京100141
相关基金：公益性行业（农业）科研专项（201503108-CC-1;201203085）

作者：杨移斌[1,2,3], 胥宁[1,2,3], 董靖[1,2,3], 杨秋红[1,2,3], 刘永涛[1,2,3], 艾晓辉[1,2,3]

关键词：斑点又尾鮰, 温和气单胞菌, 分离鉴定, 药敏特性, 回归感染实验, Ictalunes punctatus , Aeromonas sobria , isolation and identification, antimicrobial susceptibility

中文摘要：

对患出血病斑点叉尾鮰进行病原分离、鉴定及药敏实验，以期为斑点叉尾鮰出血病的防控提供参考。从患病斑点叉尾鮰血水、腹水及重要组织器官中分离纯化病原菌，并进行分离株理化特性测定、gyrB序列分析及回归感染实验，并通过K—B法进行分离菌株药物敏感性分析。结果显示，回归感染实验鱼出现与自然发病类似症状，并经细菌再分离获得相同分离株，表明分离株是引发本次斑点叉尾鮰疾病的病原菌。分离株理化特性与温和气单胞菌基本一致，gyrB基因序列分析分离株与温和气单胞菌同源性达100％，故分离株鉴定为温和气单胞菌。分离株对多西环素及头孢噻肟高度敏感，对氟苯尼考、阿奇霉素及左氧氟沙星中度敏感。分离菌株是斑点叉尾鮰病原菌，紧急时可选用多西环素等药物进行防控。[中国渔业质量与标准，2017，7（4）：45—50]

英文摘要：

The present study reports isolation, identification and antibiotic sensitivity of a pathogen from Ictalurus punctatu suffering from bleeding disorder. The pathogenic bacteria were isolated and purified from blood and important tissues and organs of Ictalurus punctatu. The identification of the isolated was conducted, using the biochemical iden- tification and 16S rRNA gene sequence determination. The artificial infection test was performed, and antimicrobial susceptibility test was conducted by K- B method. The bacterial strains isolated from artificial infection fish showed similar morphological and biochemical characteristics to those naturally infected fish. Therefore, the isolate was patho- genic bacteria giving rise to Ictalunes punctatus. According to morphological and biochemical characteristics as well as the result of gyrB gene sequence analysis, the isolate was Aeromonas sobria. The isolate was susceptible to doxycycline and cefotaxime. Meanwhile, it showed medium sensitivity to florfenicol, azithromycin and levofloxacin. Our results demonstrated that isolate was the pathogen which caused high mortality in Ictalurus punctatus, and the disease may be prevented by administering drugs such as doxycycline in emergency. [ Chinese Fishery Quality and Standards, 2017, 7（4）：45 -50]

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