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中文摘要：
目的探讨微RNA（miRNA）-21对人肺癌H358细胞体外增殖及诱导凋亡方面的作用。方法采用体外转染法将miRNA-21抑制剂瞬时转染H358细胞48h后,应用定量real-time PCR特异探针法检测H358细胞中miRNA-21的表达水平,应用MTT法检测H358细胞的生长情况,荧光缺口末端标记法（TUNEL）法分析H358细胞凋亡情况,并用Western blot检测H358细胞中抑癌基因PTEN表达的变化。结果与对照组比较,miRNA-21抑制剂转染组H358细胞的miRNA-21表达水平显著降低（P〈0.05）,细胞生长明显受到抑制（P〈0.05）,且通过PTEN靶蛋白诱导细胞凋亡（P〈0.05）。结论低表达miRNA-21可以抑制人肺癌H358细胞的体外增殖,诱导细胞凋亡,为肺癌生物治疗提供新的治疗靶点。
英文摘要：
Objective To study the effects of microRNA-21 on the proliferation and apoptosis of human lung cancer cell line H358.Methods MiRNA-21 inhibitor was transiently transferred into human lung cancer cell line H358 by using transfection in vitro.The expression level of miRNA-21 in H358cells was detected by real-time PCR before or after transfection.The MTT assay was used to assess the proliferation of H358 cells.And the cell apoptosis was analyzed by TUNEL Apoptosis Assay Kit.Furthermore,the expression of phosphatase and tensin homolog deleted on chromosome ten（PTEN）was determined by the Western-blot assay.Results The expression of miRNA-21 was markedly decreased compared with the control group（P〈0.05）.The proliferation of miRNA-21inhibitor-tranfected H358 cells was obviously inhibited（P〈0.01）.And expression level of miRNA-21 in H358cells was significantly decreased,the cell growth was significantly inhibited,moreover apoptosis was induced by PTEN target protein（P〈0.05）.Conclusion Low expression of miRNA-21 could inhibit the in vitro proliferation of H358 cells and induces apoptosis,which could provide a novel therapeutic target for anti-cancer biotherapy.
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