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Anticancer Effect of Total Annonaceous Acetogenins on Hepatocarcinoma

ISSN号：1006-446X
期刊名称：《广东微量元素科学》
时间：0
分类：Q949.747.3[生物学—植物学] S896.6[农业科学—特种经济动物饲养;农业科学—畜牧兽医]
作者机构：[1]Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing (100193), China, [2]Henan Institute of Engineering, Zhengzhou (451191 ), China
相关基金：Supported by National Mega-project for Innovative Drugs （No. 2012ZX09301002-001-026 and 2009ZX09103-390） and Beijing Natural Science Foundation （No. 7122112）

作者：杨润梅[1], 李雯敏[1], 胡卫军[2], 黄文华[1], 朱春燕[1], 余竞光[1], 赵欣[1], 蔡大勇[1], 高南南[1]

关键词：番荔枝内酯, 抗癌作用, 肝癌, BEL-7402细胞, BEL-7402细胞, 线粒体膜电位, 细胞周期阻滞, 流式细胞仪, annonaceous acetogenins, hepatocarcinoma, proliferation, apoptosis

中文摘要：

目的将证实从 Annona squamosa 悬崖提炼的全部的 annonaceous acetogenins (TAA ) 的 anticancer 效果。在人的 hepatocarcinoma 上。TAA 的禁止的效果在 H ₂₂-bearing 老鼠被表明的方法。TAA 的力量作为它的 50% 被证实禁止集中(在染色的硫玫瑰精 B 下面的 Bel-7402 房间上的 IC ₅₀) 。两个都位于机制下面在流动 cytometry 下面作为细胞的 apoptosis 和房间周期被探索。Mitochondrial 和接受者 apoptotic 小径在荧光分析下面在流动 cytometry 和 caspases 活动下面作为 mitochondrial 膜潜力被区分。结果在老鼠的 TAA 的禁止的率在 4 mg/kg 剂量是 50.98% 。Bel-7402 上的 TAA 的 IC ₅₀ 是 20.06 ? 硥散摥 ?? ‰潭 ?? 敬 ? 潴挠瑹瑯硯捩瑩 ? 桷牥慥? 潣据湥牴瑡潩獮戠汥睯ㄠ‰浮汯? 猠潨敷 ? 潮猠杩楮楦慣瑮攠晦 ' ?獡挠浯慰敲 ?楷桴琠敨挠湯牴汯

英文摘要：

Objective： To confirm the anticancer effect of total annonaceous acetogenins （TAAs） abstracted from Annona squamosa Linn. on human hepatocarcinoma. Methods： The inhibitory effect of TAAs was demonstrated in H,,-bearing mice. The potency of TAAs was confirmed as its 50% inhibiting concentration （IC50） on Bel-7402 cell under Sulfur Rhodamine B staining. Both underlying mechanisms were explored as cellular apoptosis and cell cycle under flow cytometry. Mitochondrial and recipient apoptotic pathways were differentiated as mitochondrial membrane potential under flow cytometry and caspases activities under fluorescence analysis. Results： The inhibitory rate of TAAs in mice was 50.98% at 4 mg/kg dose. The IC50 of TAAs on Bel-7402 was 20.06 μg/mL （15.13-26.61 μg/mL）. Effective mechanisms of TAAs were confirmed as both of arresting cell cycle at G1 phase and inducing apoptosis dose- and time-dependently. Mitochondrial and recipient pathways involved in apoptotic actions of TAAs. Conclusion： TAAs is effective for hepatocarcinoma, via inhibiting proliferation and inducing apoptosis.

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