为研究长江口中华鲟幼鱼盐度适应过程及其调节规律,将7月龄幼鱼直接转入0(淡水对照)、5、10和15等4个盐度组中养殖32d,分别在第0.5、1、2、4、8、16和32天检测幼鱼血液水分、血清渗透压和血清Na+、Cl-、K+的浓度,结果显示,实验过程中,淡水和盐度5组中华鲟幼鱼血液各项指标始终保持一致,未呈现显著性差异。盐度10和15组,中华鲟幼鱼血液水分含量呈先下降后上升趋势,下降程度与盐度呈正相关,16d时各组幼鱼血液水分无显著性差异。中华鲟幼鱼转入盐度10和15条件下,其血清渗透压与Na+和Cl-浓度的变化趋势一致,表现为先上升后下降,最后达到新的平衡;而血清K+浓度的变化趋势与血清渗透压和Na+、Cl-离子不同,呈现先下降后趋于平稳的趋势。研究表明,中华鲟幼鱼与其他广盐性鱼类一样,其盐度适应过程可分为2个阶段,即临界期和调整期。
To understand salinity adaptation and regulatory status of juvenile Chinese sturgeon in the Yangtze Estuary,the 7-month-old juveniles were transferred directly from freshwater(0)to salinity 5,10, and 15 for 32 days. And the blood moisture content,serum osmolality and ion concentrations of fish exposed to various salinities were measured respectively at 0.5,1,2,4,8,16, and 32 days after transfer. Results showed that:all measured blood parameters of juveniles in freshwater control and salinity 5 kept the same pattern, and those are not significantly different. In the salinity 10 and 15 groups,blood moisture content of juveniles decreased first,and then increased. The decrements were positively correlated to salinities, and the blood moisture content of juveniles in all groups was not significantly different at 16 days after transfer. Changes in serum osmolality and Na+, C1- concentrations of juveniles exposed to salinity 10 and 15 were consistent, i. e. increased significantly,then decreased and reached a new steady state. However, change in concentration of serum K+ in juveniles exposed to salinity 10 and 15 differed from the serum osmolality and Na+ , C1- concentrations. It decreased and reached a new stable state. The results indicated that salinity acclimation process of juvenile Chinese sturgeon,like other euryhaline fishes,can be divided into two periods:the critical period and the regulatory period.