欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
采用模拟运输的方法,选取平均体长为(13.58±0.23)cm、平均体质量为(8.55±0.39)g的长江刀鲚幼鱼为实验对象,设置正常应激组和加盐抗应激组(盐度为10‰),每组3个平行,运输结束后将剩余的鱼放回原培育池,研究分析了正常应激组和加盐抗应激组在装载前(BL)、装载后(AL)及运输胁迫2h、4h、6h、8h和恢复24h、96h后,长江刀鲚血浆渗透压、皮质醇、血糖和肝糖原的变化规律及恢复情况。结果显示,正常应激组和加盐抗应激组经装载、8h运输及恢复96h后的成活率分别为20%和100%。运输胁迫导致正常应激组刀鲚血液渗透压整体呈下降趋势,10‰盐度则显著提高血浆渗透压,至运输8h后,血液渗透压达到最高值(0.348±0.002)m Osm/kg。长江刀鲚血浆皮质醇在运输2h后急剧升高达到最大值,而10‰盐度使得运输刀鲚的血浆皮质醇在运输4h后达到峰值(574.71±64.75)ng/m L。运输胁迫导致正常应激组长江刀鲚血糖的明显升高;而加盐抗应激组血糖含量的变化幅度显著低于正常应激组,运输6h后血糖值趋于稳定。肝糖原的变化规律与血糖浓度相对应,推测血糖值的变化主要源于肝脏糖原的动员。实验结果表明,10‰盐度可显著提高血浆渗透压水平,降低其能量物质消耗,避免了撞壁、擦伤掉鳞等强烈的应激反应,显著提高了成活率。
英文摘要：
In this study, we selected Coilia nasus from the Yangtze River with the average body length of (13.58±0.23) cm and the average body weight of (8.55±0.39) g as the experimental subjects, and we simulated the conditions during transportation for the experiments. These fish were divided into the normal stress group and the salt stress group (with 10 ‰ salinity), and there were three parallel repeats in each group. After the 8h transportation the survivors were put back into the original breeding pools. Samples from both groups were collected at certain time points-before loading (BL), after loading (AL), 2h, 4h, 6h, and 8h after the beginning of transportation, 24h recovery, and 96h recovery. We tested the plasma osmotic pressure, cortisol, glucose and the liver glycogen of these samples. The result showed that after the 96h recovery, the survival rates of the normal stress group and the salt stress group were 20% and 100% re-spectively. The blood osmotic pressure was reduced in the normal stress group after transportation. In contrast it was significantly elevated in the salt stress group, and reached to the peak value [(0.348±0.002) mOsm/kg] 8h after the be-ginning of transportation. The plasma cortisol rapidly climbed to the peak 2h after the beginning of transportation in the normal stress group, whereas, it reached to the peak [(574.71±64.75) ng/mL] 4h after the beginning of transportation in the salt stress group. The level of glucose in the normal stress group was significantly elevated after the transportation, but its change in the salt stress group was much milder and the concentration was stabilized 6h after the beginning of transportation. Moreover, the glucose concentration in the salt stress group was significantly lower than that in the nor-mal stress group. The change in the liver glycogen was consistent with the change in glucose level, so we speculated that the increase in glucose resulted from the glycogenolysis of the liver glycogen. These results suggested that 10‰salinity could signific
同期刊论文项目
同项目期刊论文
期刊信息
位置：