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中文摘要：
利用RT-PCR和RACE技术获得刀鲚（Coilia nasus）Δ6脂肪酸去饱和酶（Δ6 fatty acyl desaturase,FAD6）的全长cDNA序列2 032 bp,开放阅读框（ORF）为1 338 bp,编码445个氨基酸。其具有3个保守的组氨酸簇（HDXGH、HXXHH和QXXHH）、2个跨膜区和含亚铁血红素结合基序（HPGG）的细胞色素b5结构域等典型的Δ6脂肪酸去饱和酶结构特点。氨基酸同源性分析显示,刀鲚FAD6与海鳗（Muraenesox cinereus）和军曹鱼（Rachycentron canadum）等海水鱼类的相似性达75%～79%,而与淡水鱼类的相似性较低。系统进化分析也显示,其与海水鱼类的亲缘关系较近。荧光定量PCR检测发现该基因在刀鲚脑和肠中高表达,肌肉和肝脏相对高表达,心脏、肾脏和鳃低表达。
英文摘要：
The full-length cDNA of Δ6 fatty acyl desaturases（FAD6） of Coilia nasus,which was involved in the biosynthesis of highly unsaturated fatty acids（HUFA）,was cloned by RT-PCR（reverse transcription polymerase chain reaction） and RACE（rapidamplification of cDNA ends） methods. The results revealed that the FAD6 cDNA was 2 032 bp,including an ORF（open reading frame） of 1 338 bp specified a protein of 445 amino acids,which contained three histidine-rich regions and two putative transmembrane domains. The deduced amino acid sequence shared 75%-79% identity with that in Muraenesox cinereus and Rachycentron canadum,and low similarity of freshwater fish. Actually,it was consistent with the results of phylogenetic analysis. The tissue-specific expressions of FAD6 mRNAs were detected in C. nasus. A high expression was observed in the brain and intestine, while relatively high expression was encountered in muscle and liver,and a weak expression in the heart,kidney and gill.
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