2012年1-4 月,在长江河口靖江段采集了一批溯河洄游中的日本鳗鲡幼鱼,对其中5尾幼鱼的矢耳石进行了全耳石SRXRF分析。结果表明,SRXRF分析可精确测得14 种元素的含量,其中钙(Ca)和锶(Sr)的平均含量分别达240 215.928 μg/g和2 383.221 μg/g,是常量元素;其余12 种均属于微量元素,含量从高至低依次为钡 (Ba)、铁(Fe)、锰(Mn)、锌(Zn)、铬(Cr)、镍(Ni)、汞(Hg)、硒(Se)、钴(Co)、铜(Cu)、金(Au)、钒(V)。其中,钡(Ba)、铁(Fe)、锰(Mn)、锌(Zn)的平均含量在1~10 μg/g之间;铬(Cr)、镍(Ni)、汞(Hg)、硒(Se)、钴(Co)、铜(Cu)、金(Au)、钒(V)的平均含量在1 μg/g以下。从元素的空间分布及全部测试点的含量分析来看,矢耳石内钙(Ca)和锶(Sr)的变异系数仅为10.11 %和21.83 %,是含量最稳定的元素;锰(Mn)、锌(Zn)、铬(Cr)、镍(Ni)、钴(Co)、铜(Cu)的变异系数在23.11 %~35.88 %之间;钒(V)、汞(Hg)、铜(Au)和钡(Ba)的变异系数均超过50%;硒(Se)的变异系数达134.31 %,是含量最不稳定的元素。研究还发现,日本鳗鲡幼体从海洋到淡水洄游的过程中,耳石中锶(Sr)与钙(Ca)的比值变化存在较长时间的时迟现象。
Japanese eel larvae Anguilla japonica were collected in Jingjiang section of Yangtze River Estuary from January to April, 2012. The elements of sagittal otolith extracted from five larval eels were detected by using synchrotron radiation X ray fluorescence (SRXRF). Results showed that fourteen elements could be accurately detected. It included two major elements Ca and Sr, the average contens of which in otolith could reach 240 215.928 μg/g and 2 383.221 μg/g respectively. The other twelve were recognized as minor elements. Their contents sequentially decreased in the following order: Ba, Fe, Mn, Zn, Cr, Ni, Hg, Se, Co, Cu, Au, V. The average concentrations of Ba, Fe, Mn, Zn were 1-10 μg/g and the average concentrations of the other elements Cr, Ni, Hg, Se, Co, Cu, Au, V were less than 1 μg/g. According to spatial distribution and the content variation of all measured elements, Ca and Sr were the most stable elements whose coefficient of variation were 10.11% and 21.83%.By comparison, the coefficient of variation of Mn, Zn, Cr, Ni, Co varied from 23.11% to 35.88% and V, Hg, Au, Ba even exceeded 50%. The most unsteady element was Se for its coefficient of variation reached 134.31%. We also found that the variation of Sr/Ca ratios might be delayed in the otolith when Japanese eels larvae migrated from sea to freshwater.