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中文摘要：
β-伴大豆球蛋白是引起过敏现象发生的主要大豆蛋白过敏原之一，建立β-伴大豆球蛋白过敏原的检测为研究大豆中过敏原降低程度奠定了良好的基础。采用自制的兔抗β-伴大豆球蛋白多克隆抗体，并以标准β-伴大豆球蛋白为抗原、辣根过氧化物酶标记的羊抗兔IgG为酶标二抗，建立了定量检测β-伴大豆球蛋白的间接竞争ELISA方法。并对该方法进行了条件优化及精密度的测定。结果为：抗原包被浓度为0.3μg/mL，一抗最佳稀释度为1∶3200，酶标二抗稀释度为1∶10000；板内误差2.19%，板间误差9.61%。回归直线方程为y=-2.4392x-1.8718，相关系数R2=0.9957，其检测的线性范围为0.1~2μg/mL。表明所建方法具有一定的重复性和灵敏度，可用于大豆制品过敏原的检测。
英文摘要：
Many researches have found thatβ-conglycinin is one of the major allergenic proteins in soybean protein allergy. Therefore,the establishment of detection system forβ-conglycinin laid good foundation in the practice of preventing soybean protein allergy. An indirect competitive ELISA for detectionβ-conglycinin was developed in this paper. Results showed that the optimal concentrationa of the coating antigen,homemade polyclonal antibody anti-β-conglycinin and horseradish peroxidase conjugated goat anti-rabbit IgG were 0.3μg/mL,1∶3200 and 1∶10000 respectively. The coefficients of variation of intra-plate error and inter-plates error were 2.19%and 9.61%. As the standard curve was obtained with regression equation as y=-2.439 2x-1.871 8（R2=0.995 7）,the linear range was 0.1~2.0μg/mL. The method has certain reproducibility and good sensitivity and can be used to detect the allergy in soybean products.
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