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中文摘要：
综合微流控技术与激光技术,对流体环境中细胞在激光作用下的运动情况进行了受力分析,并对其作用机理进行了研究。首先针对不同物理尺寸的细胞在侧向激光作用下的运动轨迹进行仿真,并根据仿真结果来优化设计芯片的微通道结构,提高其分离效率。同时在制作微流控芯片的过程中,将光纤直接固定在模板上用PDMS进行浇注,这样得到的芯片可以直接将激光引入,形成侧向激光对通道内细胞进行干预,并使其运动轨迹发生改变。实验结果表明,在鞘流和细胞液分别以0.2μL/min和0.1μL/min的速度进样时,使用960 nm,200 mW的泵浦激光可以使细胞轨迹发生偏移。实验结果与仿真结果基本相符。
英文摘要：
By integrating the microfluidic technology and laser technology,the force analysis on movement of cells in flowing fluid environment under the action of the laser was carried out,and its mechanism of the action was researched.Firstly,the movement trajectories of the cells with different physical sizes under the action of the lateral direction laser were simulated,then according to the simulation results,the design of the chip micro-channel structure was optimized,and the separation efficiency was improved.At the same time in the production process of the microfluidic chip,the PDMS was cast on the templates with the optical fiber,thus the laser was introduced directly into the chip,and the lateral laser was formed to intervene and change the trajectory of the cells in channel.The experimental results indicate that when the injection speeds of the sheath flow and cell liquid are 0.2μL/min and 0.1μL/min,respectively,the cell track can be shifted using 960 nm,200 mW pump laser.The experimental results are consistent with the simulation results.
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