研究黄河三角洲滨海湿地先锋植物盐地碱蓬在水盐胁迫下的生存机制能够为退化滨海湿地的修复提供重要的理论基础.通过温室控制模拟试验,研究了不同地下水位深度(0、-10、-20、-30 cm)以及不同盐浓度(0%、1%、2%、3%)的交互作用对盐地碱蓬幼苗高度、分枝数、生物量以及叶绿素含量和抗氧化物酶的活性的影响.结果表明,从地下水位深度-30~0 cm,盐地碱蓬幼苗高度显著降低,分枝数以及根、茎、叶生物量均显著减少;不同盐浓度对幼苗高度,分枝数以及生物量影响也差异显著.在-30 cm水位和0%盐的交互作用下幼苗总生物量达到最高,为(1.09±0.15)g/株,而0 cm水位深度和3%盐胁迫的交互作用下其总生物量为(0.23±0.01)g/株,仅为最高生物量的21%.同样地,在-30 cm水位和0%盐的交互作用下幼苗叶片叶绿素a、b以及类胡萝卜素含量最高,而0 cm水位深度和3%盐胁迫的交互作用下各含量达到最低.超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性随盐浓度增加而显著增加,以0 cm水位为例,SOD活性从55.00 U/mg(0%NaCl)增加到151.58 U/mg(3%NaCl);当地下水位下降时,SOD活性下降,但CAT活性在-30 cm水位深度时最高;在0和-10 cm水位下,丙二醛(MDA)含量随盐浓度增加而增加,-30 cm水位下,MDA含量在3%盐浓度时为0.26 mmol/g,仅为其他盐浓度下的28%~40%.以上结果表明盐地碱蓬能够通过改变形态特征、生物量分配比例以及调节体内抗氧化胁迫物质含量来适应不同的生存环境,这正是盐地碱蓬在滨海湿地潮间带不同水盐环境中能够长期生存的适应性机制.
The halophyte Suaeda salsa is the pioneer plant and is used for the degraded coastal wetland in Yellow River Delta.The water-salt stress is the most important factor for ecological restoration to degraded coastal wetland.To understand the adaptive mechanism of Suaeda salsa to water-salt stresses,the induced effects of different groundwater table depths(0,-10,-20,-30 cm) and salt stress(0%,1%,2%,3%) on seedlings of Suaeda salsa plant were characterized by the growth parameters of plant height,branch number and biomass of different organs and biological indices of leaf chlorophyll content,the activities of SOD,CAT,the leaf content of MDA and protein.The results showed the significantly(p0.001) decreased height of the seedlings from-30 cm to 0 cm of groundwater table depth,together with the decreased the number of branches,the biomass of leaf,shoot and root.The highest total biomass of single plant was(1.09±0.15) g under the condition of-30 cm water table depth and 0% salt stress.However,the combination of 0 cm water table depth and 3% NaCl resulted in the biomass of(0.23±0.01) g,which was ca.21% compared with the highest biomass.Similarly,the contents of leaf chlorophyll a,b and carotenoid were the highest under the condition of-30 cm water table depth and 0% salt stress and lowest under the condition of 0 cm water table depth combined with 3% NaCl.The activities of SOD,CAT were increased significantly(p0.05) depending on the increase of salt stress.At 0 cm water table depth,the activities of SOD were 55.00 U/mg with 0% NaCl and 151.58 U/mg with 3% NaCl,respectively.The activities of SOD were decreased when the water table depth increased.However,the activities of CAT achieved the highest level at-30 cm water table depth.At 0 and-10 cm water table depth,the MDA content increased with the increase of salt stress.The MDA content was 0.26 mmol/g at-30 cm water table depth with 3% NaCl,which was approx.28%-40% of the MDA contents compared with that caused by other salt stresses.These results d