在温室土培条件下,研究了不同K肥用量对长春花盛花期的生物量、抗氧化物酶活性、丙二醛含量、叶绿素含量及文多灵、长春质碱、长睿碱和长春新碱等生物碱含量的影响。结果表明:当外源KCI浓度为100—400mg/kg时可以有效促进长春花的生长,且超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性随KCI浓度增加而逐渐升高,叶绿素含量也逐渐升高,但叶片丙二醛(MDA)含量则显著下降。当外源KCI浓度达800mg/kg时,过量的K营养供应抑制了长春花的正常生长。400mg/kg的施K量可最大限度地促进盛花期长春花的正常生长,并显著促进其生物碱的代谢,使得长春花盛花期文多灵、长春质碱、长眷碱和长春新碱含量显著高于其他所有处理。含量分别达42.81、37.82、1.88和3.1mg/g。
The effects of different K fertilizer rates on the biomass, the activites of antioxidant enzymes, the MDA content, the chlorophyll content and the contents of vindoline,catharanthine,vinblastine and vincristine of flowering Catharanthus roseus were investigated in greenhouse. The results indicated that the KCl could promote the growth of C. roseus when it was 100 ~ 400 mg/kg. The activities of SOD, CAT and POD enzymes and the content of chlorophyll were gradually increased,but the MDA content was decreased with the increase of KCl concentration. The excess of K nutrition could inhibit the growth of C. roseus when the KCl was 800 mg/kg. The uses of 400 mg/kg KCl could significantly promote the growth and the alkaloids metabolism of flowering C. roseus. Also the vindoline, catharanthine, vinblastine and vincristine contents were highest when the KCl was 400 mg/kg,with 42.81, 37.82, 1.88 and 3.1 mg/g, respectively.