中文摘要：

目的探讨抗巨噬细胞移动抑制因子（MIF）单抗对溃疡性结肠炎（UC）小鼠治疗效果及炎症因子水平的影响。方法选取30只雄性BALB/c小鼠,分为正常对照组、UC模型组、抗MIF单抗组各10只,以5%葡聚糖硫酸钠诱导建立小鼠UC模型,建模后抗MIF单抗组采用抗MIF单抗腹腔注射给药。给药期间观察各组小鼠一般情况并进行疾病活动指数（DAI）评分、大肠大体损伤评分及组织学评分。建模后第8天检测血清MIF、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）浓度。结果与正常对照组比较,UC模型组及抗MIF单抗组小鼠DAI评分、大肠大体损伤评分及组织学评分均明显增高（P〈0.05）;与UC模型组比较,抗MIF单抗组DAI评分、大肠大体损伤评分及组织学评分均明显下降（P〈0.05）;与正常对照组相比,UC模型组及抗MIF单抗组MIF、TNF-α和IL-6浓度显著升高（P〈0.05）;与UC模型组相比,抗MIF单抗组MIF、TNF-α和IL-6浓度降低（P〈0.05）。结论抗MIF单抗能有效地抑制UC小鼠血清炎症因子的水平,对UC的治疗有一定的效果。

英文摘要：

Objective To investigate the effects of anti-macrophage migration inhibitory factor（ MIF） antibody（ Ab） on efficacy and inflammatory factors in mice with ulcerative colitis（ UC）. Methods Thirty BALB / c male mice were enrolled and divided into normal control group,UC model group and anti-MIF Ab group,with 10 mice in each group. UC model was established using 5% dextran sulfate sodium in mice. After the modeling,the anti-MIF Ab group was administered transabdominally the anti-MIF Ab. During the period of drug administration,the general situation,disease activity index（ DAI） score,gross injury score and histological score of large intestine were evaluated in each group. On the 8th day after modeling,the serum concentrations of MIF,tumor necrosis factor-（ TNF-α）,interleukin-6（ IL-6） were detected. Results Compared to the normal control group,the DAI score,gross injury score and histological score of large intestine were significantly higher in the UC model group or anti-MIF Ab group（ P〈0. 05）. Compared to the UC model group,the DAI score,gross injury score and histological score of large intestine were significantly lower in the anti-MIF Ab group（ P〈0. 05）. Compared to the normal control group,the concentrations of MIF,TNF-α and IL-6 remarkably increased in the UC model group or anti-MIF Ab group（ P〈0. 05）. Compared to the UC model group,the concentrations of MIF,TNF-α and IL-6 decreased in the anti-MIF Ab group（ P〈0. 05）.Conclusion Anti-MIF Ab has a strongly inhibitory effect on the levels of serum inflammatory factors in mice with UC and is effective on the treatment of UC to a degree.