采用不同剂量的UV—B辐射极地雪藻,测定了致死率、色素含量、蛋白质和总脂含量以及自由基清除活性的变化.结果表明,UV—B辐射对极地雪藻具有一定的致死效应,UV-B辐射后雪藻细胞的叶绿素和类胡萝卜素含量增加,虾青素含量也显著提高.UV—B辐射后,极地雪藻基本生化成分的含量发生了较为明显的变化:蛋白质含量随辐射时间的延长而逐渐降低,总脂含量则逐步增加.经UV—B辐射培养以后,极地雪藻在有机溶剂系统中的自由基清除活性有所提高.
Studied in this paper are some changes of lethal rate, pigment content, protein content, total lipid content and scavenging activity on radicals of Chlamydomonas nivalis exposed to UV-B with different dosages. The resuits show that UV-B radiation is lethal for Chlamydomonas nivalis, and that the contents of chlorophyll, carotenoid and astaxanthin increase after the radiation. Moreover, the basic biochemical components of Chlamydomonas nivalis obviously change after the UV-B radiation, that is, the protein content decreases, the total lipid content increases, and the scavenging activity on radicals of Chlamydomonas nivalis in organic solvent improves.