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中文摘要：
以白骨壤树枝为材料，经提取、分离和纯化等一系列步骤得到一中性多糖组分HAM-3-IIa，经高效凝胶过滤色谱（HPGFC）和凝胶柱层析Sephacryl（S-400）鉴定为均一组分，其重均相对分子质量（Mw）为97600。化学组成分析表明，HAM-3-IIa为中性糖蛋白，含蛋白4．9％，含多糖95．0％；由阿拉伯糖（Ara）、半乳糖（Gal）和木糖（Xyl）组成；β-消去反应说明HAM-3-IIa中糖链以O-糖肽键连接到苏氨酸上的；补体结合实验表明，该多糖通过经典途径和替代途径共同作用产生显著的抗补体活性，呈一定的剂量效应关系，并研究确定该活性不是由于纯化过程中所产生的微生物所造成的。
英文摘要：
HAM-3-IIa, a neutral polysaccharide fraction, was obtained from Avicennia marina（ Forsk. ） Vierh. by a series steps of extraction, isolation and purification. The purified HAM-3-IIa was proved to be homogenous by HPGFC and gel column chromatography. Its molecular weight was 97 600. HAM-3-IIa was regarded as neutral glycoprotein with 4.9 % protein and 95 % carbohydrate. The carbohydrate fraction was composed of arabinose, galactose and xylose. Linkage between sugars and amino acids was shown to be O-glycol-Ser bond by β-elimination reactions. HAM-3-IIa expressed potent anti-complementary activity via classical and alternative pathways with the increase of activity proportional to dosage. It was confirmed from the study that the activity of polysaccharide was not due to the presence of microbial contamination during the purification processes.
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