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中文摘要：
为预防与控制大黄鱼溃疡病的发生,本研究利用特异多重PCR技术,对象山县养殖大黄鱼的3种致病弧菌感染情况进行了流行病学调查。每月在各定点网箱组随机采集大黄鱼肝、肾、脾、肌肉等组织进行感染情况的检测。结果显示,3种致病弧菌对大黄鱼的感染率存在一定的差异性。溶藻弧菌、哈维弧菌对大黄鱼的感染率整体高于副溶血弧菌;从感染组织分析,与肝脏、肾脏相比3种致病弧菌更易感染肌肉、脾脏;3种致病弧菌对不同鱼龄的大黄鱼感染率各不相同。研究还表明,3种致病弧菌在各个采样时间点均有感染,其中7—9月为弧菌感染高峰期,而台风后的感染率较台风前都有一定提高。另外,根据大黄鱼溃疡病发生与3种致病弧菌感染率关系的研究可知,在大黄鱼溃疡病暴发前几天,弧菌的感染率都会显示出较高数值。因此,可以通过3种致病弧菌对大黄鱼感染的分子流行病学调查与分析来预测预警病害的发生和流行。
英文摘要：
In order to prevent and control the incidence of ulcer in large yellow croaker,we evaluated the prevalence of infection with three pathogenic vibrios in cultured Larimichthys crocea in Xiangshan by specific multiplex PCR. We collected the liver,kidney, spleen,muscle of fish in each sentinel cage randomly and detected the infection status. The results showed that there were some differences between the three kinds of pathogenic vibrios that infected the fish. The infection rate of V. alginolyticus and V. harvey large in L. crocea was higher than V. parahaemolyticus. The three vibrios were more susceptible to muscle, spleen than to the liver and kidney. The infection rate of different age of fish was not identical. In every sampling time, there existed infection rate. From July to September was the peak of infection rate and the rate increased after the typhoon certainly. In addition, according to the investigation on the relationship between incidence of ulcer and the prevalence of pathogenic vibrio,it showed that the infection rate would reach a higher value a few days before the outbreak of ulcer disease. So we could forecast the incidence and prevalence of the diseases through molecular epidemiology investigation and analyzing the infection of three pathogenic vibrios in the fish. Then, we will establish RT-PCR method of three vibrios to analyse the intensity of infection further. Thus the experimental results will be more convincing.
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