跟踪检测6种海洋微藻在生长过程中单位细胞β-葡聚糖的含量变化,分析其累积时期、累积速率以及提取制备的得率和纯度,并对几种微藻来源的β-葡聚糖进行免疫活性检测。结果表明:β-葡聚糖在微藻种群增殖的平台期累积量最大,不同种类的微藻细胞累积β-葡聚糖能力差异较大。假微型海链藻(Thalassiosirapseudonana)具有较大的累积速率、提取制备得率以及较大的免疫活性。6种海洋微藻来源的β-葡聚糖都显示比酵母来源的β-葡聚糖免疫活性高。
The accumulation features of microalgal fl-glucans, including accumulation phase and rate, have been characterized by monitoring the content of β-glucans from six marine microalgae during their growth cycles. The extraction and immuno-stimulative effects of β-glucans were also studied. The results indicated that the content of cellular β-glucans reached the maximum level at stationary phase for all microalgae, however, with large variation depending on species. Among six microalgae, Thalassiosira pseudonana showed outstanding features for its β-glucans with high accumulation rate, isolation yield, and significant immuno-stimulative effects. Therefore, the immuno-stimulative effects of β-glucans were higher from microalgae than that from yeast.