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中文摘要：
为了解越冬期间水温下降对大黄鱼抗氧化水平和血清酶活性的影响，作者研究了养殖大黄鱼（Pseudosciaena crocea）冬季海区自然降温不同阶段（20、16、12、10、8℃）肝脏和肌肉中抗氧化水平及血清酶活性的变化。结果显示：自然降温过程中，肝脏中3种抗氧化酶变化趋势不同，其中SOD活性呈升高趋势，8℃时SOD活性最强；POD活性呈先升高后降低的变化趋势，16℃时活性最高；CAT活性呈下降趋势；而肌肉中这3种抗氧化酶活性均呈下降趋势。肝脏中GSH和MDA含量在16℃时最高；肌肉中的3种抗氧化酶活性、GSH和MDA含量以及总抗氧化能力（T-AOC）均明显低于肝脏中，反映了肝脏对清除自由基具有重要作用，在抗氧化调节方面起到主要作用。血清酶ALT、AST、ALP、LDH及CK-MB随水温自然下降酶活性均呈现降低趋势，即20℃时酶活性最强；CK和LIP随着水温降低，呈先上升再下降的趋势：ADA和GGT随水温降低活性呈上升趋势。血清酶的不同变化，说明水温对大黄鱼血清酶活性有一定的影响。
英文摘要：
In order to investigate the effects of natural water cooling on antioxidant level and serum enzyme activity in farmed large yellow croaker （Pseudosciaena crocea） in winter, the changes of antioxidant level and serum en- zyme activity were examined at different stages of natural cooling （20℃, 16℃, 12℃, 10℃, and 8℃）. The results showed that in liver, the activity of SOD exhibited an increase trend with the highest activity at 8℃ ; the activity of POD increased first and then decreased, with the highest activity at 16℃; the activity of CAT showed a decrease trend. While in muscle, the activities of these three antioxidant enzymes all decreased during natural water cooling. In liver, the highest concentration of GSH and MDA and the maximum value of total antioxidant capacity （T-AOC） were all observed at 16℃. The activity of SOD, POD and CAT, the concentration of GSH and MDA and the value of T-AOC in liver, were all higher than those in muscle, suggesting the critical roles of liver in free radical scavenging and antioxidation regulation. The activity of serum enzymes （ALT, AST, ALP, LDH and CK-MB） all showed a de- clining trend, and the highest activities at 20℃. The activity of CK and LIP increased first and then decreased with decreased water temperature, while ADA and GGT revealed an increasing trend. These data suggest that water temperature impacts on the serum enzyme activities in P crocea.
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