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中文摘要：
为研究I型鸭肝炎病毒（DHV-1）在鸭胚成纤维细胞系（DEF-h）中的增殖规律,本研究将DHV-1野毒株（ZJ-08株）接种DEF-h细胞,连续传代3次后,观察病毒对细胞的致病变效应（CPE）,并对病毒核酸及其蛋白的表达和病毒增殖特性进行了鉴定。研究结果显示,DHV ZJ-08株能够在DEF-h中有效增殖,并产生典型的CPE,病毒蛋白在细胞中也获得了良好表达,并与DHV-1特异性抗体发生反应。一步生长曲线显示ZJ-08株病毒感染DEF-h 12 h开始增殖,36 h～60 h进入快速增殖期,72 h达到峰值,TCID50为10-4.3/mL。本实验为进一步的DHV-1的致病机理和细胞灭活疫苗的研制奠定了基础。
英文摘要：
To explore the proliferation characteristics of duck hepatitis virus type 1 （DHV-1） in duck embryo fibroblast cell line （DEF-h）, DEF-h cells were inoculated with DHV-1 ZJ-08 strain. After passaged three times, CPE could be observed clearly at 36 hpi. The results of RT-PCR, IFA and western blot showed that virual proteins of DHV-1 were expressed well in DEF-h cells, and could reacted to the specific antibody against DHV-I. One step growth curve revealed that DHV-1 began to proliferate at 12 hpi, and reached a rapid proliferation stage at 36 hpi. The virus titre of DHV-1 reached peak at 72 hpi. This study provide some valuable information for further exploring the pathogenesis mechanism of DHV-1 and the vaccine production.
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